Insulin-induced GLUT4 translocation involves protein kinase C-λ-mediated functional coupling between Rab4 and the motor protein kinesin

Takeshi Imamura, Jie Huang, Isao Usui, Hiroaki Satoh, Jennie Bever, Jerrold M. Olefsky

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

Insulin stimulates glucose transport by promoting translocation of GLUT4 proteins from the perinuclear compartment to the cell surface. It has been previously suggested that the microtubule-associated motor protein kinesin, which transports cargo toward the plus end of microtubules, plays a role in translocating GLUT4 vesicles to the cell surface. In this study, we investigated the role of Rab4, a small GTPase-binding protein, and the motor protein KIF3 (kinesin II in mice) in insulin-induced GLUT4 exocytosis in 3T3-L1 adipocytes. Photoaffinity labeling of Rab4 with [y-32P] GTP-azidoanilide showed that insulin stimulated Rab4 GTP loading and that this insulin effect was inhibited by pretreatment with the phosphatidylinositol 3-kinase (PI3-kinase) inhibitor LY294002 or expression of dominant-negative protein kinase C-λ (PKC-λ). Consistent with previous reports, expression of dominant-negative Rab4 (N121I) decreased insulin-induced GLUT4 translocation by 45%. Microinjection of an anti-KIF3 antibody into 3T3-L1 adipocytes decreased insulin-induced GLUT4 exocytosis by 65% but had no effect on endocytosis. Coimmunoprecipitation experiments showed that Rab4, but not Rab5, physically associated with KIF3, and this was confirmed by showing in vitro association using glutathione S-transferase-Rab4. A microtubule capture assay demonstrated that insulin stimulation increased the activity for the binding of KIF3 to microtubules and that this activation was inhibited by pretreatment with the PI3-kinase inhibitor LY294002 or expression of dominant-negative PKC-λ. Taken together, these data indicate that (i) insulin signaling stimulates Rab4 activity, the association of Rab4 with kinesin, and the interaction of KIF3 with microtubules and (ii) this process is mediated by insulin-induced PI3-kinase-dependent PKC-λ activation and participates in GLUT4 exocytosis in 3T3-L1 adipocytes.

Original languageEnglish (US)
Pages (from-to)4892-4900
Number of pages9
JournalMolecular and Cellular Biology
Volume23
Issue number14
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

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rab4 GTP-Binding Proteins
Kinesin
Protein Kinase C
Insulin
Phosphatidylinositol 3-Kinase
Microtubules
Exocytosis
Adipocytes
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Glucose Transporter Type 4
Microtubule-Associated Proteins
Monomeric GTP-Binding Proteins
Microinjections
Endocytosis
Guanosine Triphosphate
Glutathione Transferase

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Insulin-induced GLUT4 translocation involves protein kinase C-λ-mediated functional coupling between Rab4 and the motor protein kinesin. / Imamura, Takeshi; Huang, Jie; Usui, Isao; Satoh, Hiroaki; Bever, Jennie; Olefsky, Jerrold M.

In: Molecular and Cellular Biology, Vol. 23, No. 14, 01.07.2003, p. 4892-4900.

Research output: Contribution to journalArticle

Imamura, Takeshi ; Huang, Jie ; Usui, Isao ; Satoh, Hiroaki ; Bever, Jennie ; Olefsky, Jerrold M. / Insulin-induced GLUT4 translocation involves protein kinase C-λ-mediated functional coupling between Rab4 and the motor protein kinesin. In: Molecular and Cellular Biology. 2003 ; Vol. 23, No. 14. pp. 4892-4900.
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AU - Huang, Jie

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AU - Bever, Jennie

AU - Olefsky, Jerrold M.

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