Instillation versus inhalation of multiwalled carbon nanotubes

Exposure-related health effects, clearance, and the role of particle characteristics

Rona M. Silva, Kyle Doudrick, Lisa M. Franzi, Christel Teesy, Donald S. Anderson, Zheqiong Wu, Somenath Mitra, Vincent Vu, Gavin Dutrow, James E. Evans, Paul Westerhoff, Laura S. Van Winkle, Otto G. Raabe, Kent E. Pinkerton

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Inhaled multiwalled carbon nanotubes (MWCNTs) may cause adverse pulmonary responses due to their nanoscale, fibrous morphology and/or biopersistance. This study tested multiple factors (dose, time, physicochemical characteristics, and administration method) shown to affect MWCNT toxicity with the hypothesis that these factors will influence significantly different responses upon MWCNT exposure. The study is unique in that (1) multiple administration methods were tested using particles from the same stock; (2) bulk MWCNT formulations had few differences (metal content, surface area/functionalization); and (3) MWCNT retention was quantified using a specialized approach for measuring unlabeled MWCNTs in rodent lungs. Male Sprague-Dawley rats were exposed to original (O), purified (P), and carboxylic acid functionalized (F) MWCNTs via intratracheal instillation and inhalation. Blood, bronchoalveolar lavage fluid (BALF), and lung tissues were collected at postexposure days 1 and 21 for quantifying biological responses and MWCNTs in lung tissues by programmed thermal analysis. At day 1, MWCNT instillation produced significant BALF neutrophilia and MWCNT-positive macrophages. Instilled O- and P-MWCNTs produced significant inflammation in lung tissues, which resolved by day 21 despite MWCNT retention. MWCNT inhalation produced no BALF neutrophilia and no significant histopathology past day 1. However, on days 1 and 21 postinhalation of nebulized MWCNTs, significantly increased numbers of MWCNT-positive macrophages were observed in BALF. Results suggest (1) MWCNTs produce transient inflammation if any despite persistence in the lungs; (2) instilled O-MWCNTs cause more inflammation than P- or F-MWCNTs; and (3) MWCNT suspension media produce strikingly different effects on physicochemical particle characteristics and pulmonary responses.

Original languageEnglish (US)
Pages (from-to)8911-8931
Number of pages21
JournalACS Nano
Volume8
Issue number9
DOIs
StatePublished - Sep 23 2014

Fingerprint

Multiwalled carbon nanotubes (MWCN)
respiration
clearances
health
carbon nanotubes
Health
lungs
macrophages
Fluids
Macrophages
fluids
Tissue
rodents
causes

Keywords

  • engineered nanomaterial
  • inflammation
  • inhalation exposure
  • multiwalled carbon nanotube (MWCNT)
  • pulmonary toxicity

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Silva, R. M., Doudrick, K., Franzi, L. M., Teesy, C., Anderson, D. S., Wu, Z., ... Pinkerton, K. E. (2014). Instillation versus inhalation of multiwalled carbon nanotubes: Exposure-related health effects, clearance, and the role of particle characteristics. ACS Nano, 8(9), 8911-8931. https://doi.org/10.1021/nn503887r

Instillation versus inhalation of multiwalled carbon nanotubes : Exposure-related health effects, clearance, and the role of particle characteristics. / Silva, Rona M.; Doudrick, Kyle; Franzi, Lisa M.; Teesy, Christel; Anderson, Donald S.; Wu, Zheqiong; Mitra, Somenath; Vu, Vincent; Dutrow, Gavin; Evans, James E.; Westerhoff, Paul; Van Winkle, Laura S.; Raabe, Otto G.; Pinkerton, Kent E.

In: ACS Nano, Vol. 8, No. 9, 23.09.2014, p. 8911-8931.

Research output: Contribution to journalArticle

Silva, RM, Doudrick, K, Franzi, LM, Teesy, C, Anderson, DS, Wu, Z, Mitra, S, Vu, V, Dutrow, G, Evans, JE, Westerhoff, P, Van Winkle, LS, Raabe, OG & Pinkerton, KE 2014, 'Instillation versus inhalation of multiwalled carbon nanotubes: Exposure-related health effects, clearance, and the role of particle characteristics', ACS Nano, vol. 8, no. 9, pp. 8911-8931. https://doi.org/10.1021/nn503887r
Silva, Rona M. ; Doudrick, Kyle ; Franzi, Lisa M. ; Teesy, Christel ; Anderson, Donald S. ; Wu, Zheqiong ; Mitra, Somenath ; Vu, Vincent ; Dutrow, Gavin ; Evans, James E. ; Westerhoff, Paul ; Van Winkle, Laura S. ; Raabe, Otto G. ; Pinkerton, Kent E. / Instillation versus inhalation of multiwalled carbon nanotubes : Exposure-related health effects, clearance, and the role of particle characteristics. In: ACS Nano. 2014 ; Vol. 8, No. 9. pp. 8911-8931.
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