Inhibition of starch synthesis results in overproduction of lipids in Chlamydomonas reinhardtii

Yantao Li, Danxiang Han, Guangrong Hu, Milton Sommerfeld, Qiang Hu

Research output: Contribution to journalArticle

238 Citations (Scopus)

Abstract

Starch and neutral lipids are two major carbon storage compounds in many microalgae and plants. Lipids are more energy rich and have often been used as food and fuel feedstocks. Genetic engineering of the lipid biosynthesis pathway to overproduce lipid has achieved only limited success. We hypothesize that through blocking the competing pathway to produce starch, overproduction of neutral lipid may be achieved. This hypothesis was tested using the green microalga Chlamydomonas reinhardtii and its low starch and starchless mutants. We discovered that a dramatic increase in neutral lipid content and the neutral lipid/total lipid ratio occurred among the mutants under high light and nitrogen starvation. BAFJ5, one of the mutants defective in the small subunit of ADP-glucose pyrophosphorylase, accumulated neutral and total lipid of up to 32.6% and 46.4% of dry weight (DW) or 8- and 3.5-fold higher, respectively, than the wild-type. These results confirmed the feasibility of increasing lipid production through redirecting photosynthetically assimilated carbon away from starch synthesis to neutral lipid synthesis. However, some growth impairment was observed in the low starch and starchless mutants, possibly due to altered energy partitioning in PSII, with more excitation energy dissipated as heat and less to photochemical conversion. This study demonstrated that biomass and lipid production by the selected mutants can be improved by physiological manipulation.

Original languageEnglish (US)
Pages (from-to)258-268
Number of pages11
JournalBiotechnology and Bioengineering
Volume107
Issue number2
DOIs
StatePublished - Oct 1 2010

Fingerprint

Chlamydomonas reinhardtii
Starch
Lipids
Glucose-1-Phosphate Adenylyltransferase
Carbon
Microalgae
Administrative data processing
Genetic engineering
Genetic Engineering
Excitation energy
Biosynthesis
Starvation
Biomass
Feedstocks
Glucose
Nitrogen

Keywords

  • Biofuel
  • High light
  • Lipid
  • Microalgae
  • Nitrogen starvation
  • Starch

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Inhibition of starch synthesis results in overproduction of lipids in Chlamydomonas reinhardtii. / Li, Yantao; Han, Danxiang; Hu, Guangrong; Sommerfeld, Milton; Hu, Qiang.

In: Biotechnology and Bioengineering, Vol. 107, No. 2, 01.10.2010, p. 258-268.

Research output: Contribution to journalArticle

Li, Yantao ; Han, Danxiang ; Hu, Guangrong ; Sommerfeld, Milton ; Hu, Qiang. / Inhibition of starch synthesis results in overproduction of lipids in Chlamydomonas reinhardtii. In: Biotechnology and Bioengineering. 2010 ; Vol. 107, No. 2. pp. 258-268.
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