Inhibition of macrophage activation by the myxoma virus M141 protein (vCD200)

Leiliang Zhang, Marianne Stanford, Jia Liu, Catherine Barrett, Lei Jiang, A. Neil Barclay, Grant McFadden

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The M141 protein of myxoma virus (MYXV) is a viral CD200 homolog (also called vOX-2) that inhibits macrophage activation in infected rabbits. Here, we show that murine myeloid RAW 264.7 cells became activated when infected with MYXV in which the M141 gene was deleted (vMyx-M141KO) but not with the parental wild-type MYXV. Moreover, transcript and protein levels of tumor necrosis factor and granulocyte colony-stimulating factor were rapidly upregulated in an NF-κB-dependent fashion in the RAW 264.7 cells infected with vMyx-M141KO. M141 protein is present in the virion and counteracts this NF-κB activation pathway upon infection with the wild-type MYXV. Our data suggest that upregulation of these classic macrophage-related proinflammatory cytokine markers following infection of myeloid cells with the M141-knockout MYXV is mediated via the rapid activation of the cellular NF-κB pathway.

Original languageEnglish (US)
Pages (from-to)9602-9607
Number of pages6
JournalJournal of virology
Volume83
Issue number18
DOIs
StatePublished - Sep 2009

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Inhibition of macrophage activation by the myxoma virus M141 protein (vCD200)'. Together they form a unique fingerprint.

Cite this