Inhibition of macrophage activation by the myxoma virus M141 protein (vCD200)

Leiliang Zhang, Marianne Stanford, Jia Liu, Catherine Barrett, Lei Jiang, A. Neil Barclay, Douglas McFadden

Research output: Contribution to journalArticle

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Abstract

The M141 protein of myxoma virus (MYXV) is a viral CD200 homolog (also called vOX-2) that inhibits macrophage activation in infected rabbits. Here, we show that murine myeloid RAW 264.7 cells became activated when infected with MYXV in which the M141 gene was deleted (vMyx-M141KO) but not with the parental wild-type MYXV. Moreover, transcript and protein levels of tumor necrosis factor and granulocyte colony-stimulating factor were rapidly upregulated in an NF-κB-dependent fashion in the RAW 264.7 cells infected with vMyx-M141KO. M141 protein is present in the virion and counteracts this NF-κB activation pathway upon infection with the wild-type MYXV. Our data suggest that upregulation of these classic macrophage-related proinflammatory cytokine markers following infection of myeloid cells with the M141-knockout MYXV is mediated via the rapid activation of the cellular NF-κB pathway.

Original languageEnglish (US)
Pages (from-to)9602-9607
Number of pages6
JournalJournal of Virology
Volume83
Issue number18
DOIs
StatePublished - Sep 1 2009
Externally publishedYes

Fingerprint

Myxoma virus
macrophage activation
Macrophage Activation
Myeloid Cells
Proteins
proteins
granulocyte colony-stimulating factor
tumor necrosis factors
Granulocyte Colony-Stimulating Factor
cells
Infection
virion
infection
Virion
macrophages
cytokines
Up-Regulation
Tumor Necrosis Factor-alpha
rabbits
Macrophages

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Zhang, L., Stanford, M., Liu, J., Barrett, C., Jiang, L., Barclay, A. N., & McFadden, D. (2009). Inhibition of macrophage activation by the myxoma virus M141 protein (vCD200). Journal of Virology, 83(18), 9602-9607. https://doi.org/10.1128/JVI.01078-09

Inhibition of macrophage activation by the myxoma virus M141 protein (vCD200). / Zhang, Leiliang; Stanford, Marianne; Liu, Jia; Barrett, Catherine; Jiang, Lei; Barclay, A. Neil; McFadden, Douglas.

In: Journal of Virology, Vol. 83, No. 18, 01.09.2009, p. 9602-9607.

Research output: Contribution to journalArticle

Zhang, L, Stanford, M, Liu, J, Barrett, C, Jiang, L, Barclay, AN & McFadden, D 2009, 'Inhibition of macrophage activation by the myxoma virus M141 protein (vCD200)', Journal of Virology, vol. 83, no. 18, pp. 9602-9607. https://doi.org/10.1128/JVI.01078-09
Zhang L, Stanford M, Liu J, Barrett C, Jiang L, Barclay AN et al. Inhibition of macrophage activation by the myxoma virus M141 protein (vCD200). Journal of Virology. 2009 Sep 1;83(18):9602-9607. https://doi.org/10.1128/JVI.01078-09
Zhang, Leiliang ; Stanford, Marianne ; Liu, Jia ; Barrett, Catherine ; Jiang, Lei ; Barclay, A. Neil ; McFadden, Douglas. / Inhibition of macrophage activation by the myxoma virus M141 protein (vCD200). In: Journal of Virology. 2009 ; Vol. 83, No. 18. pp. 9602-9607.
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