Inhibition of macrophage activation by calcium channel blockers and calmodulin antagonists

B. Wright, I. Zeidman, R. Greig, G. Poste

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

The biochemical mechanisms by which macrophages become activated to the tumoricidal state are poorly understood. To investigate the role of calcium in this process, the effect of calcium channel blockers and calmodulin antagonists on the acquisition of tumoricidal properties by macrophages activated by a number of different agents was examined. Activation of thioglycollate-stimulated C57BL/6 mouse peritoneal macrophages by macrophage activation factor (MAF) plus LPS, IFN-γ plus LPS or the calcium ionophore, A23187, was inhibited in a doseependent fashion by the calcium channel blockers nifedipine and verapamil. These agents blocked the influx of 45Ca into macrophages activated by MAF plus LPS. Macrophage activation was also inhibited by chlorpromazine, W-7, and calmidazolium at concentrations known to perturb calmodulin function. The data suggest that activation of macrophages to the tumoricidal state is a calcium-dependent process involving the participation of calcium-regulated biochemical reactions whose activities can be modulated by pharmacological agents that frustrate transmembrane calcium fluxes and/or inhibit calmodulin function.

Original languageEnglish (US)
Pages (from-to)46-53
Number of pages8
JournalCellular Immunology
Volume95
Issue number1
DOIs
StatePublished - Oct 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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