Inhibition of expression of virulence genes of Yersinia pestis in Escherichia coli by external guide sequences and RNase P

Jae Hyeong Ko, Mina Izadjoo, Sidney Altman

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

External guide sequences (EGSs) targeting virulence genes from Yersinia pestis were designed and tested in vitro and in vivo in Escherichia coli. Linear EGSs and M1 RNA-linked EGSs were designed for the yscN and yscS genes that are involved in type III secretion in Y. pestis. RNase P from E. coli cleaves the messages of yscN and yscS in vitro with the cognate EGSs, and the expression of the EGSs resulted in the reduction of the levels of these messages of the virulence genes when those genes were expressed in E. coli.

Original languageEnglish (US)
Pages (from-to)1656-1662
Number of pages7
JournalRNA
Volume14
Issue number8
DOIs
StatePublished - Aug 1 2008
Externally publishedYes

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Keywords

  • Pathogenic bacteria
  • Random EGSs

ASJC Scopus subject areas

  • Molecular Biology

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