Inhibition of Chemokine-Glycosaminoglycan interactions in donor tissue reduces mouse allograft vasculopathy and transplant rejection

Erbin Dai, Li Ying Liu, Hao Wang, Dana McIvor, Yun Ming Sun, Colin Macaulay, Elaine King, Ganesh Munuswamy-Ramanujam, Mee Yong Bartee, Jennifer Williams, Jennifer Davids, Israel Charo, Douglas McFadden, Jeffrey D. Esko, Alexandra R. Lucas

Research output: Contribution to journalArticle

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Abstract

Background: Binding of chemokines to glycosaminoglycans (GAGs) is classically described as initiating inflammatory cell migration and creating tissue chemokine gradients that direct local leukocyte chemotaxis into damaged or transplanted tissues. While chemokine-receptor binding has been extensively studied during allograft transplantation, effects of glycosaminoglycan (GAG) interactions with chemokines on transplant longevity are less well known. Here we examine the impact of interrupting chemokine-GAG interactions and chemokine-receptor interactions, both locally and systemically, on vascular disease in allografts. Methodology/Principal Findings: Analysis of GAG or CC chemokine receptor 2 (CCR2) deficiency were coupled with the infusion of viral chemokine modulating proteins (CMPs) in mouse aortic allograft transplants (n = 239 mice). Inflammatory cell invasion and neointimal hyperplasia were significantly reduced in N-deacetylase-N-sulfotransferase-1 (Ndst1f/fTekCre+) heparan sulfate (GAG)-deficient (Ndst1-/-, p<0.044) and CCR2-deficient (Ccr2-/-, p<0.04) donor transplants. Donor tissue GAG or CCR2 deficiency markedly reduced inflammation and vasculopathy, whereas recipient deficiencies did not. Treatment with three CMPs was also investigated; Poxviral M-T1 blocks CC chemokine receptor binding, M-T7 blocks C, CC, and CXC GAG binding, and herpesviral M3 binds receptor and GAG binding for all classes. M-T7 reduced intimal hyperplasia in wild type (WT) (Ccr2+/+, p≤0.003 and Ccr2-/-, p≤0.027) aortic allografts, but not in Ndst1-/- aortic allografts (p = 0.933). M-T1 and M3 inhibited WT (Ccr2+/+ and Ndst1+/+, p≤0.006) allograft vasculopathy, but did not block vasculopathy in Ccr2-/- (p = 0.61). M-T7 treatment alone, even without immunosuppressive drugs, also significantly prolonged survival of renal allograft transplants (p≤0.001). Conclusions/Significance: Interruption of chemokine-GAG interactions, even in the absence of chemokine-receptor blockade, is a highly effective approach to reduction of allograft rejection, reducing vascular inflammation and prolonging allograft survival. Although chemokines direct both local and systemic cell migration, interruption of inherent chemokine responses in the donor tissue unexpectedly had a greater therapeutic impact on allograft vasculopathy.

Original languageEnglish (US)
Article numbere10510
JournalPLoS One
Volume5
Issue number5
DOIs
StatePublished - Sep 14 2010
Externally publishedYes

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graft rejection
Transplants
allografting
glycosaminoglycans
Graft Rejection
chemokines
Glycosaminoglycans
Chemokines
Allografts
Tissue Donors
Tissue
mice
CCR2 Receptors
Chemokine Receptors
cell movement
hyperplasia
Cell Movement
Hyperplasia
inflammation
CCR Receptors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Inhibition of Chemokine-Glycosaminoglycan interactions in donor tissue reduces mouse allograft vasculopathy and transplant rejection. / Dai, Erbin; Liu, Li Ying; Wang, Hao; McIvor, Dana; Sun, Yun Ming; Macaulay, Colin; King, Elaine; Munuswamy-Ramanujam, Ganesh; Bartee, Mee Yong; Williams, Jennifer; Davids, Jennifer; Charo, Israel; McFadden, Douglas; Esko, Jeffrey D.; Lucas, Alexandra R.

In: PLoS One, Vol. 5, No. 5, e10510, 14.09.2010.

Research output: Contribution to journalArticle

Dai, E, Liu, LY, Wang, H, McIvor, D, Sun, YM, Macaulay, C, King, E, Munuswamy-Ramanujam, G, Bartee, MY, Williams, J, Davids, J, Charo, I, McFadden, D, Esko, JD & Lucas, AR 2010, 'Inhibition of Chemokine-Glycosaminoglycan interactions in donor tissue reduces mouse allograft vasculopathy and transplant rejection', PLoS One, vol. 5, no. 5, e10510. https://doi.org/10.1371/journal.pone.0010510
Dai, Erbin ; Liu, Li Ying ; Wang, Hao ; McIvor, Dana ; Sun, Yun Ming ; Macaulay, Colin ; King, Elaine ; Munuswamy-Ramanujam, Ganesh ; Bartee, Mee Yong ; Williams, Jennifer ; Davids, Jennifer ; Charo, Israel ; McFadden, Douglas ; Esko, Jeffrey D. ; Lucas, Alexandra R. / Inhibition of Chemokine-Glycosaminoglycan interactions in donor tissue reduces mouse allograft vasculopathy and transplant rejection. In: PLoS One. 2010 ; Vol. 5, No. 5.
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abstract = "Background: Binding of chemokines to glycosaminoglycans (GAGs) is classically described as initiating inflammatory cell migration and creating tissue chemokine gradients that direct local leukocyte chemotaxis into damaged or transplanted tissues. While chemokine-receptor binding has been extensively studied during allograft transplantation, effects of glycosaminoglycan (GAG) interactions with chemokines on transplant longevity are less well known. Here we examine the impact of interrupting chemokine-GAG interactions and chemokine-receptor interactions, both locally and systemically, on vascular disease in allografts. Methodology/Principal Findings: Analysis of GAG or CC chemokine receptor 2 (CCR2) deficiency were coupled with the infusion of viral chemokine modulating proteins (CMPs) in mouse aortic allograft transplants (n = 239 mice). Inflammatory cell invasion and neointimal hyperplasia were significantly reduced in N-deacetylase-N-sulfotransferase-1 (Ndst1f/fTekCre+) heparan sulfate (GAG)-deficient (Ndst1-/-, p<0.044) and CCR2-deficient (Ccr2-/-, p<0.04) donor transplants. Donor tissue GAG or CCR2 deficiency markedly reduced inflammation and vasculopathy, whereas recipient deficiencies did not. Treatment with three CMPs was also investigated; Poxviral M-T1 blocks CC chemokine receptor binding, M-T7 blocks C, CC, and CXC GAG binding, and herpesviral M3 binds receptor and GAG binding for all classes. M-T7 reduced intimal hyperplasia in wild type (WT) (Ccr2+/+, p≤0.003 and Ccr2-/-, p≤0.027) aortic allografts, but not in Ndst1-/- aortic allografts (p = 0.933). M-T1 and M3 inhibited WT (Ccr2+/+ and Ndst1+/+, p≤0.006) allograft vasculopathy, but did not block vasculopathy in Ccr2-/- (p = 0.61). M-T7 treatment alone, even without immunosuppressive drugs, also significantly prolonged survival of renal allograft transplants (p≤0.001). Conclusions/Significance: Interruption of chemokine-GAG interactions, even in the absence of chemokine-receptor blockade, is a highly effective approach to reduction of allograft rejection, reducing vascular inflammation and prolonging allograft survival. Although chemokines direct both local and systemic cell migration, interruption of inherent chemokine responses in the donor tissue unexpectedly had a greater therapeutic impact on allograft vasculopathy.",
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T1 - Inhibition of Chemokine-Glycosaminoglycan interactions in donor tissue reduces mouse allograft vasculopathy and transplant rejection

AU - Dai, Erbin

AU - Liu, Li Ying

AU - Wang, Hao

AU - McIvor, Dana

AU - Sun, Yun Ming

AU - Macaulay, Colin

AU - King, Elaine

AU - Munuswamy-Ramanujam, Ganesh

AU - Bartee, Mee Yong

AU - Williams, Jennifer

AU - Davids, Jennifer

AU - Charo, Israel

AU - McFadden, Douglas

AU - Esko, Jeffrey D.

AU - Lucas, Alexandra R.

PY - 2010/9/14

Y1 - 2010/9/14

N2 - Background: Binding of chemokines to glycosaminoglycans (GAGs) is classically described as initiating inflammatory cell migration and creating tissue chemokine gradients that direct local leukocyte chemotaxis into damaged or transplanted tissues. While chemokine-receptor binding has been extensively studied during allograft transplantation, effects of glycosaminoglycan (GAG) interactions with chemokines on transplant longevity are less well known. Here we examine the impact of interrupting chemokine-GAG interactions and chemokine-receptor interactions, both locally and systemically, on vascular disease in allografts. Methodology/Principal Findings: Analysis of GAG or CC chemokine receptor 2 (CCR2) deficiency were coupled with the infusion of viral chemokine modulating proteins (CMPs) in mouse aortic allograft transplants (n = 239 mice). Inflammatory cell invasion and neointimal hyperplasia were significantly reduced in N-deacetylase-N-sulfotransferase-1 (Ndst1f/fTekCre+) heparan sulfate (GAG)-deficient (Ndst1-/-, p<0.044) and CCR2-deficient (Ccr2-/-, p<0.04) donor transplants. Donor tissue GAG or CCR2 deficiency markedly reduced inflammation and vasculopathy, whereas recipient deficiencies did not. Treatment with three CMPs was also investigated; Poxviral M-T1 blocks CC chemokine receptor binding, M-T7 blocks C, CC, and CXC GAG binding, and herpesviral M3 binds receptor and GAG binding for all classes. M-T7 reduced intimal hyperplasia in wild type (WT) (Ccr2+/+, p≤0.003 and Ccr2-/-, p≤0.027) aortic allografts, but not in Ndst1-/- aortic allografts (p = 0.933). M-T1 and M3 inhibited WT (Ccr2+/+ and Ndst1+/+, p≤0.006) allograft vasculopathy, but did not block vasculopathy in Ccr2-/- (p = 0.61). M-T7 treatment alone, even without immunosuppressive drugs, also significantly prolonged survival of renal allograft transplants (p≤0.001). Conclusions/Significance: Interruption of chemokine-GAG interactions, even in the absence of chemokine-receptor blockade, is a highly effective approach to reduction of allograft rejection, reducing vascular inflammation and prolonging allograft survival. Although chemokines direct both local and systemic cell migration, interruption of inherent chemokine responses in the donor tissue unexpectedly had a greater therapeutic impact on allograft vasculopathy.

AB - Background: Binding of chemokines to glycosaminoglycans (GAGs) is classically described as initiating inflammatory cell migration and creating tissue chemokine gradients that direct local leukocyte chemotaxis into damaged or transplanted tissues. While chemokine-receptor binding has been extensively studied during allograft transplantation, effects of glycosaminoglycan (GAG) interactions with chemokines on transplant longevity are less well known. Here we examine the impact of interrupting chemokine-GAG interactions and chemokine-receptor interactions, both locally and systemically, on vascular disease in allografts. Methodology/Principal Findings: Analysis of GAG or CC chemokine receptor 2 (CCR2) deficiency were coupled with the infusion of viral chemokine modulating proteins (CMPs) in mouse aortic allograft transplants (n = 239 mice). Inflammatory cell invasion and neointimal hyperplasia were significantly reduced in N-deacetylase-N-sulfotransferase-1 (Ndst1f/fTekCre+) heparan sulfate (GAG)-deficient (Ndst1-/-, p<0.044) and CCR2-deficient (Ccr2-/-, p<0.04) donor transplants. Donor tissue GAG or CCR2 deficiency markedly reduced inflammation and vasculopathy, whereas recipient deficiencies did not. Treatment with three CMPs was also investigated; Poxviral M-T1 blocks CC chemokine receptor binding, M-T7 blocks C, CC, and CXC GAG binding, and herpesviral M3 binds receptor and GAG binding for all classes. M-T7 reduced intimal hyperplasia in wild type (WT) (Ccr2+/+, p≤0.003 and Ccr2-/-, p≤0.027) aortic allografts, but not in Ndst1-/- aortic allografts (p = 0.933). M-T1 and M3 inhibited WT (Ccr2+/+ and Ndst1+/+, p≤0.006) allograft vasculopathy, but did not block vasculopathy in Ccr2-/- (p = 0.61). M-T7 treatment alone, even without immunosuppressive drugs, also significantly prolonged survival of renal allograft transplants (p≤0.001). Conclusions/Significance: Interruption of chemokine-GAG interactions, even in the absence of chemokine-receptor blockade, is a highly effective approach to reduction of allograft rejection, reducing vascular inflammation and prolonging allograft survival. Although chemokines direct both local and systemic cell migration, interruption of inherent chemokine responses in the donor tissue unexpectedly had a greater therapeutic impact on allograft vasculopathy.

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