Influence of substrate C/N ratio on the structure of multi-species biofilms consisting of nitrifiers and heterotrophs

A. Ohashi, D. G V De Silva, B. Mobarry, J. A. Manem, D. A. Stahl, Bruce Rittmann

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

A bench-scale biological aerated filter (BAF) was operated under a range of substrate loadings and C/N influent ratios. Start-up with a substrate loading of 4.8 COD kg m-3 d-1 and an influent C/N ratio of 1.5 gave no nitrification, due to the high biofilm detachment rate caused by the daily backwashing. Subsequent runs with lower substrate loadings and backwashing rates allowed stable nitrification. A final run with the initial substrate loading and backwash rate gave stable nitrification. Oligonucleotide probes targeted to the 16S rRNA of the nitrifiers, as well as to other types of microorganisms, were used to characterize how the changes in loading and detachment affected the microbial structure in the biofilms. The proportion of nitrifiers decreased with increasing C/N ratio. The biofilm remaining after backwashing also contained a higher fraction of nitrifiers than did the backwash solids stripped from the outer layers of biofilm, which consisted mainly of active heterotrophs. Thus, stable nitrification was possible only when the nitrifiers became established deeper inside the biofilm.

Original languageEnglish (US)
Pages (from-to)75-84
Number of pages10
JournalWater Science and Technology
Volume32
Issue number8
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Biofilms
Nitrification
biofilm
nitrification
substrate
Substrates
Oligonucleotides
Microorganisms
microorganism
probe
filter
rate

Keywords

  • 16S rRNA
  • Biofilm
  • Biological aerated filter
  • C/N ratio
  • Heterotrophs
  • Nitrification
  • Nitrifiers
  • Oligonucleotide probe

ASJC Scopus subject areas

  • Water Science and Technology

Cite this

Influence of substrate C/N ratio on the structure of multi-species biofilms consisting of nitrifiers and heterotrophs. / Ohashi, A.; De Silva, D. G V; Mobarry, B.; Manem, J. A.; Stahl, D. A.; Rittmann, Bruce.

In: Water Science and Technology, Vol. 32, No. 8, 1995, p. 75-84.

Research output: Contribution to journalArticle

Ohashi, A. ; De Silva, D. G V ; Mobarry, B. ; Manem, J. A. ; Stahl, D. A. ; Rittmann, Bruce. / Influence of substrate C/N ratio on the structure of multi-species biofilms consisting of nitrifiers and heterotrophs. In: Water Science and Technology. 1995 ; Vol. 32, No. 8. pp. 75-84.
@article{3b2ce0eb9055401ebcde023d3f3ba31c,
title = "Influence of substrate C/N ratio on the structure of multi-species biofilms consisting of nitrifiers and heterotrophs",
abstract = "A bench-scale biological aerated filter (BAF) was operated under a range of substrate loadings and C/N influent ratios. Start-up with a substrate loading of 4.8 COD kg m-3 d-1 and an influent C/N ratio of 1.5 gave no nitrification, due to the high biofilm detachment rate caused by the daily backwashing. Subsequent runs with lower substrate loadings and backwashing rates allowed stable nitrification. A final run with the initial substrate loading and backwash rate gave stable nitrification. Oligonucleotide probes targeted to the 16S rRNA of the nitrifiers, as well as to other types of microorganisms, were used to characterize how the changes in loading and detachment affected the microbial structure in the biofilms. The proportion of nitrifiers decreased with increasing C/N ratio. The biofilm remaining after backwashing also contained a higher fraction of nitrifiers than did the backwash solids stripped from the outer layers of biofilm, which consisted mainly of active heterotrophs. Thus, stable nitrification was possible only when the nitrifiers became established deeper inside the biofilm.",
keywords = "16S rRNA, Biofilm, Biological aerated filter, C/N ratio, Heterotrophs, Nitrification, Nitrifiers, Oligonucleotide probe",
author = "A. Ohashi and {De Silva}, {D. G V} and B. Mobarry and Manem, {J. A.} and Stahl, {D. A.} and Bruce Rittmann",
year = "1995",
doi = "10.1016/0273-1223(96)00010-8",
language = "English (US)",
volume = "32",
pages = "75--84",
journal = "Water Science and Technology",
issn = "0273-1223",
publisher = "IWA Publishing",
number = "8",

}

TY - JOUR

T1 - Influence of substrate C/N ratio on the structure of multi-species biofilms consisting of nitrifiers and heterotrophs

AU - Ohashi, A.

AU - De Silva, D. G V

AU - Mobarry, B.

AU - Manem, J. A.

AU - Stahl, D. A.

AU - Rittmann, Bruce

PY - 1995

Y1 - 1995

N2 - A bench-scale biological aerated filter (BAF) was operated under a range of substrate loadings and C/N influent ratios. Start-up with a substrate loading of 4.8 COD kg m-3 d-1 and an influent C/N ratio of 1.5 gave no nitrification, due to the high biofilm detachment rate caused by the daily backwashing. Subsequent runs with lower substrate loadings and backwashing rates allowed stable nitrification. A final run with the initial substrate loading and backwash rate gave stable nitrification. Oligonucleotide probes targeted to the 16S rRNA of the nitrifiers, as well as to other types of microorganisms, were used to characterize how the changes in loading and detachment affected the microbial structure in the biofilms. The proportion of nitrifiers decreased with increasing C/N ratio. The biofilm remaining after backwashing also contained a higher fraction of nitrifiers than did the backwash solids stripped from the outer layers of biofilm, which consisted mainly of active heterotrophs. Thus, stable nitrification was possible only when the nitrifiers became established deeper inside the biofilm.

AB - A bench-scale biological aerated filter (BAF) was operated under a range of substrate loadings and C/N influent ratios. Start-up with a substrate loading of 4.8 COD kg m-3 d-1 and an influent C/N ratio of 1.5 gave no nitrification, due to the high biofilm detachment rate caused by the daily backwashing. Subsequent runs with lower substrate loadings and backwashing rates allowed stable nitrification. A final run with the initial substrate loading and backwash rate gave stable nitrification. Oligonucleotide probes targeted to the 16S rRNA of the nitrifiers, as well as to other types of microorganisms, were used to characterize how the changes in loading and detachment affected the microbial structure in the biofilms. The proportion of nitrifiers decreased with increasing C/N ratio. The biofilm remaining after backwashing also contained a higher fraction of nitrifiers than did the backwash solids stripped from the outer layers of biofilm, which consisted mainly of active heterotrophs. Thus, stable nitrification was possible only when the nitrifiers became established deeper inside the biofilm.

KW - 16S rRNA

KW - Biofilm

KW - Biological aerated filter

KW - C/N ratio

KW - Heterotrophs

KW - Nitrification

KW - Nitrifiers

KW - Oligonucleotide probe

UR - http://www.scopus.com/inward/record.url?scp=0029554211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029554211&partnerID=8YFLogxK

U2 - 10.1016/0273-1223(96)00010-8

DO - 10.1016/0273-1223(96)00010-8

M3 - Article

VL - 32

SP - 75

EP - 84

JO - Water Science and Technology

JF - Water Science and Technology

SN - 0273-1223

IS - 8

ER -