Influence of matrices on oxygen sensing of three sensing films with chemically conjugated platinum porphyrin probes and preliminary application for monitoring of oxygen consumption of Escherichia coli (E. coli)

Yanqing Tian, Bradley R. Shumway, Weimin Gao, Cody Youngbull, Mark R. Holl, Roger H. Johnson, Deirdre Meldrum

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Oxygen sensing films were synthesized by a chemical conjugation of functional platinum porphyrin probes in silica gel, polystyrene (PS), and poly(2-hydroxyethyl methacrylate) (PHEMA) matrices. Responses of the sensing films to gaseous oxygen and dissolved oxygen were studied and the influence of the matrices on the sensing behaviors was investigated. Silica gel films had the highest fluorescence intensity ratio from deoxygenated to oxygenated environments and the fastest response time to oxygen. PHEMA films had no response to gaseous oxygen, but had greater sensitivity and a faster response time for dissolved oxygen than those of PS films. The influence of matrices on oxygen response, sensitivity and response time was discussed. The influence is most likely attributed to the oxygen diffusion abilities of the matrices. Since the probes were chemically immobilized in the matrices, no leaching of the probes was observed from the sensing films when applied in aqueous environment. One sensing film made from the PHEMA matrix was used to preliminarily monitor the oxygen consumption of Escherichia coli (E. coli) bacteria. E. coli cell density and antibiotics ampicillin concentration dependent oxygen consumption was observed, indicating the potential application of the oxygen sensing film for biological application.

Original languageEnglish (US)
Pages (from-to)579-587
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume150
Issue number2
DOIs
StatePublished - Oct 28 2010

    Fingerprint

Keywords

  • Chemical conjugation
  • E. coli
  • Matrix influence
  • Oxygen sensor
  • Platinum porphyrin

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this