Influence of maternal antibody on efficacy of embryo vaccination with cell-associated and cell-free Marek's disease vaccine.

J. M. Sharma, C. K. Graham

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Vaccination with turkey herpesvirus (HVT) of 18-day-old chicken embryos from a commercial source or from a cross (15 X 7) of two inbred lines induced better protection against early post-hatch challenge with virulent Marek's disease virus (MDV) than vaccination at hatch, despite the presence in embryos of maternally derived antibodies to HVT or to HVT and MDV. However, 50%-protective-dose (PD50) assays revealed that maternal antibodies in embryos reduced vaccine efficacy. The PD50 assays were conducted by vaccinating 15 X 7 embryos with serial dilutions of HVT at the 18th day of incubation. Embryonally vaccinated and unvaccinated chicks were challenged with MDV on the day of hatch. In the absence of maternal antibodies, the PD50 values in plaque-forming units for cell-associated and cell-free HVT were 57 and 328, respectively. In the presence of maternal antibodies, PD50 values for cell-associated and cell-free HVT were 105 and greater than 4,000, respectively.

Original languageEnglish (US)
Pages (from-to)860-870
Number of pages11
JournalAvian Diseases
Volume26
Issue number4
StatePublished - Oct 1982
Externally publishedYes

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Marek Disease Vaccines
Marek disease
maternal immunity
Marek Disease
Mardivirus
embryo (animal)
Vaccination
Embryonic Structures
vaccination
Mothers
vaccines
Antibodies
Viruses
Meleagrid Herpesvirus 1
cells
Meleagrid herpesvirus 1
assays
inbred lines
Chickens
Vaccines

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • veterinary(all)

Cite this

Influence of maternal antibody on efficacy of embryo vaccination with cell-associated and cell-free Marek's disease vaccine. / Sharma, J. M.; Graham, C. K.

In: Avian Diseases, Vol. 26, No. 4, 10.1982, p. 860-870.

Research output: Contribution to journalArticle

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