Infection and activation of bursal macrophages by virulent infectious bursal disease virus

Mahesh Khatri, Joseph M. Palmquist, Ra Mi Cha, Jagdev M. Sharma

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

In this study the effect of infectious bursal disease virus (IBDV) on bursal macrophages during the acute phase of the infection was examined. Specific-pathogen-free (SPF) chickens were exposed to virulent IBDV and bursal adherent cells were examined by immunohistochemisrty and RT-PCR for virus infection and by real-time quantitative RT-PCR (qRT-PCR) for mRNA transcripts of proinflammatory cytokines and iNOS. Viral genome was detected in bursal macrophages at 3, 5 and 7 days post-infection (dpi). Immuno-histochemical staining revealed double positive cells for KUL01 (macrophage marker) and intracellular viral proteins, showing viral replication in bursal macrophages of infected chickens. We noted a significant decrease in the total number of bursal macrophages in infected chickens, probably due to the lysis of infected cells. However, likely due to extensive necrosis of B cells, the relative proportion of bursal macrophages was significantly higher (P < 0.05) in infected birds at 3 and 5 dpi than in controls. Among the cytokines examined, IL-6 showed the greatest upregulation (100-fold increase) at 3 dpi. Expression of IL-1β was maximum at 3 dpi whereas IL-18 expression was highest at 1 dpi. Enhanced expression of iNOS mRNA was observed at 5 dpi. Increased expression of the proinflammatory cytokines and iNOS correlated well with the presence of the inflammatory response in the infected bursa. These data suggested that B cells may not be the sole targets for the virus; macrophages and possibly other cells may serve as host for IBDV.

Original languageEnglish (US)
Pages (from-to)44-50
Number of pages7
JournalVirus Research
Volume113
Issue number1
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

Infectious bursal disease virus
Macrophage Activation
Macrophages
Infection
Chickens
Cytokines
B-Lymphocytes
Specific Pathogen-Free Organisms
Messenger RNA
Interleukin-18
Viral Genome
Viral Proteins
Virus Diseases
Infection Control
Interleukin-1
Birds
Real-Time Polymerase Chain Reaction
Interleukin-6
Necrosis
Up-Regulation

Keywords

  • Bursal macrophages
  • IBDV
  • Proinflammatory cytokines

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Infection and activation of bursal macrophages by virulent infectious bursal disease virus. / Khatri, Mahesh; Palmquist, Joseph M.; Cha, Ra Mi; Sharma, Jagdev M.

In: Virus Research, Vol. 113, No. 1, 10.2005, p. 44-50.

Research output: Contribution to journalArticle

Khatri, Mahesh ; Palmquist, Joseph M. ; Cha, Ra Mi ; Sharma, Jagdev M. / Infection and activation of bursal macrophages by virulent infectious bursal disease virus. In: Virus Research. 2005 ; Vol. 113, No. 1. pp. 44-50.
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