Induction of suppressor cells, interleukin-2 production and mitogenesis with monomeric concanavalin a: Different actions of tetrameric and monomeric concanavalin A

Don E. Griswold, Laura Antell, Paul E. Bender, Nabil Hanna, George Poste

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3 Scopus citations

Abstract

The ability of a photoalkylated monomeric concanavalin A (Con A) derivative to induce mitogenesis, interleukin-2 (IL-2) production and suppressor cells in murine spleen cell cultures has been compared with the activity of native, tetrameric Con A. The monomeric derivative was prepared by photochemically induced alkylation of tryptophan residues of tetravalent Con A in the presence of chloroacetamide followed by sizing chromatography [Tanaka et al. (1981) J. Biochem. 89, 1643-1646]. The monomeric derivative appeared to display less mitogenic activity than the tetramer and was also less effective in inducing IL-2 production. No difference was detected between the monomeric and tetrameric forms of Con A in inducing suppressor cells. The data suggest that cross-linking and bridging via sugar-binding sites, while potentiating mitogenesis and IL-2 production, had little effect on suppressor cell induction.

Original languageEnglish (US)
Pages (from-to)1311-1316
Number of pages6
JournalMolecular Immunology
Volume22
Issue number12
DOIs
StatePublished - Dec 1985
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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