Induction of rabbit cyclooxygenase 2 in the anterior uvea following glaucoma filtration surgery

Min S. Chang; James C. Tsai; Rong Yang; Raymond N. DuBois; Matthew D. Breyer; Denis M. O'Day

doi:10.1076/ceyr.16.11.1147.5107

Induction of rabbit cyclooxygenase 2 in the anterior uvea following glaucoma filtration surgery

Min S. Chang, James C. Tsai, Rong Yang, Raymond N. DuBois, Matthew D. Breyer, Denis M. O'Day

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Purpose. This study was undertaken to evaluate for the presence of cyclooxygenase 2 (COX2) gene expression in the anterior uvea of rabbits following glaucoma filtration surgery. Methods. One of the following formed on the right eye of New Zealand white albino rabbits: (1) paracentesis (2.5 mm limbal incision); (2) iridectomy through a 2.5 mm limbal incision; (3) lamellar scleral flap formation or (4) full glaucoma filtration surgery. The animals were sacrificed within 3 hours of post-surgery, and the anterior uveal tissues were isolated. Polymerase chain reaction-based techniques were employed to assay for the presence of COX2 transcript. Results. A partial coding sequence of the previously unreported rabbit COX2 gene was obtained. COX2 mRNA was detected in the operated eyes of animals that underwent either full filtration surgery or iridectomy through a limbal incision. Conclusions. In normal rabbit anterior uveal tissue, there appears to be minimal expression of COX2 message. After experimental glaucoma filtration surgery, there is rapid induction of COX2 message.

Original language	English (US)
Pages (from-to)	1147-1151
Number of pages	5
Journal	Current Eye Research
Volume	16
Issue number	11
DOIs	https://doi.org/10.1076/ceyr.16.11.1147.5107
State	Published - 1997
Externally published	Yes

Keywords

Anterior uvea
Cyclooxygenase 2
Glaucoma filtration surgery
Prostaglandins
Rabbit

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.1076/ceyr.16.11.1147.5107

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title = "Induction of rabbit cyclooxygenase 2 in the anterior uvea following glaucoma filtration surgery",

abstract = "Purpose. This study was undertaken to evaluate for the presence of cyclooxygenase 2 (COX2) gene expression in the anterior uvea of rabbits following glaucoma filtration surgery. Methods. One of the following formed on the right eye of New Zealand white albino rabbits: (1) paracentesis (2.5 mm limbal incision); (2) iridectomy through a 2.5 mm limbal incision; (3) lamellar scleral flap formation or (4) full glaucoma filtration surgery. The animals were sacrificed within 3 hours of post-surgery, and the anterior uveal tissues were isolated. Polymerase chain reaction-based techniques were employed to assay for the presence of COX2 transcript. Results. A partial coding sequence of the previously unreported rabbit COX2 gene was obtained. COX2 mRNA was detected in the operated eyes of animals that underwent either full filtration surgery or iridectomy through a limbal incision. Conclusions. In normal rabbit anterior uveal tissue, there appears to be minimal expression of COX2 message. After experimental glaucoma filtration surgery, there is rapid induction of COX2 message.",

keywords = "Anterior uvea, Cyclooxygenase 2, Glaucoma filtration surgery, Prostaglandins, Rabbit",

author = "Chang, {Min S.} and Tsai, {James C.} and Rong Yang and DuBois, {Raymond N.} and Breyer, {Matthew D.} and O'Day, {Denis M.}",

note = "Funding Information: The authors thank Dr. Alan Brash, Dr. Ellen Dees and Dr. Rick Haselton for their helpful suggestions. This research was supported by NIH grants EY01621 and DK37097 (MDB), an unrestricted grant to Prevent Blindness, and Clinical Investigator Award from Veterans Administration (MDB).",

year = "1997",

doi = "10.1076/ceyr.16.11.1147.5107",

language = "English (US)",

volume = "16",

pages = "1147--1151",

journal = "Current Eye Research",

issn = "0271-3683",

publisher = "Informa Healthcare",

number = "11",

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TY - JOUR

T1 - Induction of rabbit cyclooxygenase 2 in the anterior uvea following glaucoma filtration surgery

AU - Chang, Min S.

AU - Tsai, James C.

AU - Yang, Rong

AU - DuBois, Raymond N.

AU - Breyer, Matthew D.

AU - O'Day, Denis M.

N1 - Funding Information: The authors thank Dr. Alan Brash, Dr. Ellen Dees and Dr. Rick Haselton for their helpful suggestions. This research was supported by NIH grants EY01621 and DK37097 (MDB), an unrestricted grant to Prevent Blindness, and Clinical Investigator Award from Veterans Administration (MDB).

PY - 1997

Y1 - 1997

N2 - Purpose. This study was undertaken to evaluate for the presence of cyclooxygenase 2 (COX2) gene expression in the anterior uvea of rabbits following glaucoma filtration surgery. Methods. One of the following formed on the right eye of New Zealand white albino rabbits: (1) paracentesis (2.5 mm limbal incision); (2) iridectomy through a 2.5 mm limbal incision; (3) lamellar scleral flap formation or (4) full glaucoma filtration surgery. The animals were sacrificed within 3 hours of post-surgery, and the anterior uveal tissues were isolated. Polymerase chain reaction-based techniques were employed to assay for the presence of COX2 transcript. Results. A partial coding sequence of the previously unreported rabbit COX2 gene was obtained. COX2 mRNA was detected in the operated eyes of animals that underwent either full filtration surgery or iridectomy through a limbal incision. Conclusions. In normal rabbit anterior uveal tissue, there appears to be minimal expression of COX2 message. After experimental glaucoma filtration surgery, there is rapid induction of COX2 message.

AB - Purpose. This study was undertaken to evaluate for the presence of cyclooxygenase 2 (COX2) gene expression in the anterior uvea of rabbits following glaucoma filtration surgery. Methods. One of the following formed on the right eye of New Zealand white albino rabbits: (1) paracentesis (2.5 mm limbal incision); (2) iridectomy through a 2.5 mm limbal incision; (3) lamellar scleral flap formation or (4) full glaucoma filtration surgery. The animals were sacrificed within 3 hours of post-surgery, and the anterior uveal tissues were isolated. Polymerase chain reaction-based techniques were employed to assay for the presence of COX2 transcript. Results. A partial coding sequence of the previously unreported rabbit COX2 gene was obtained. COX2 mRNA was detected in the operated eyes of animals that underwent either full filtration surgery or iridectomy through a limbal incision. Conclusions. In normal rabbit anterior uveal tissue, there appears to be minimal expression of COX2 message. After experimental glaucoma filtration surgery, there is rapid induction of COX2 message.

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Induction of rabbit cyclooxygenase 2 in the anterior uvea following glaucoma filtration surgery

Abstract

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