Induction of alpha/beta interferon by myxoma virus is selectively abrogated when primary mouse embryo fibroblasts become immortalized

Fuan Wang, John W. Barrett, Yiyue Ma, Gregory A. Dekaban, Grant McFadden

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Mouse embryo fibroblasts (MEFs) are a widely used cell culture system in life sciences, including virology. Here, we show that although primary MEFs are nonpermissive to myxoma virus replication, the corresponding immortalized MEFs support a highly productive myxoma virus infection. We further demonstrate that this permissive phenotype for myxoma virus in immortalized MEFs is due to the immortalization-associated selective block to the cellular alpha/beta interferon induction machinery involved in responding to myxoma virus challenge. Thus, our report presents a clear example, illustrating that a drastic phenotypic alteration can occur with respect to virus infection between primary cells and their immortalized counterparts.

Original languageEnglish (US)
Pages (from-to)5928-5932
Number of pages5
JournalJournal of virology
Volume83
Issue number11
DOIs
StatePublished - Jun 1 2009

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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