Inducing β-sheets formation in synthetic spider silk fibers by aqueous post-spin stretching

Bo An, Michael B. Hinman, Gregory P. Holland, Jeffery Yarger, Randolph V. Lewis

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

As a promising biomaterial with numerous potential applications, various types of synthetic spider silk fibers have been produced and studied in an effort to produce man-made fibers with mechanical and physical properties comparable to those of native spider silk. In this study, two recombinant proteins based on Nephila clavipes Major ampullate Spidroin 1 (MaSp1) consensus repeat sequence were expressed and spun into fibers. Mechanical test results showed that fiber spun from the higher molecular weight protein had better overall mechanical properties (70 KD versus 46 KD), whereas postspin stretch treatment in water helped increase fiber tensile strength significantly. Carbon-13 solid-state NMR studies of those fibers further revealed that the postspin stretch in water promoted protein molecule rearrangement and the formation of β-sheets in the polyalanine region of the silk. The rearrangement correlated with improved fiber mechanical properties and indicated that postspin stretch is key to helping the spider silk proteins in the fiber form correct secondary structures, leading to better quality fibers.

Original languageEnglish (US)
Pages (from-to)2375-2381
Number of pages7
JournalBiomacromolecules
Volume12
Issue number6
DOIs
StatePublished - Jun 13 2011

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Silk
Stretching
Fibers
Proteins
Mechanical properties
Recombinant proteins
Water
Biocompatible Materials
Recombinant Proteins
Biomaterials
Tensile strength
Carbon
Physical properties
Molecular weight
Nuclear magnetic resonance
Molecules

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials

Cite this

Inducing β-sheets formation in synthetic spider silk fibers by aqueous post-spin stretching. / An, Bo; Hinman, Michael B.; Holland, Gregory P.; Yarger, Jeffery; Lewis, Randolph V.

In: Biomacromolecules, Vol. 12, No. 6, 13.06.2011, p. 2375-2381.

Research output: Contribution to journalArticle

An, Bo ; Hinman, Michael B. ; Holland, Gregory P. ; Yarger, Jeffery ; Lewis, Randolph V. / Inducing β-sheets formation in synthetic spider silk fibers by aqueous post-spin stretching. In: Biomacromolecules. 2011 ; Vol. 12, No. 6. pp. 2375-2381.
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