Indirect reduction of cytochrome b559 in isolated reaction centres of photosystem II by exogenous flavins

J. Klein, J. De Las Rivas, J. Barber

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Cytochrome b559 contained within the isolated reaction centre of photosystem II can, under anaerobic conditions, be directly reduced by photoinduced electron transfer from exogenously added flavins. This chlorophyll-independent reaction occurs when glucose oxidase is present. Glucose oxidase is a flavin-containing enzyme (its prosthetic group being flavin-adenine dinucleotide) and is often used as part of an oxygen trap together with glucose and catalase. Thus, studies using this chemical trap to create anaerobic conditions should take into account the possibility of inducing a non-photosynthetic electron transfer process in isolated photosystem II complexes. However, the flavin-catalyzed process is restricted to excitation with blue light and can be used as a convenient non-chemical method to modify the redox state of cytochrome b559.

Original languageEnglish (US)
Pages (from-to)9-14
Number of pages6
JournalBioelectrochemistry and Bioenergetics
Volume38
Issue number1
DOIs
StatePublished - Aug 1995
Externally publishedYes

Keywords

  • Chlorophyll
  • Cytochrome b559
  • Gel electrophoresis
  • Photosystem II

ASJC Scopus subject areas

  • Biophysics
  • Physical and Theoretical Chemistry
  • Electrochemistry

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