Independent initiation of primary electron transfer in the two branches of the photosystem I reaction center

Marc G. Müller, Chavdar Slavov, Rajiv Luthra, Kevin Redding, Alfred R. Holzwarth

    Research output: Contribution to journalArticle

    76 Citations (Scopus)

    Abstract

    Photosystem I (PSI) is a large pigment-protein complex that unites a reaction center (RC) at the core with ∼100 core antenna chlorophylls surrounding it. The RC is composed of two cofactor branches related by a pseudo-C2 symmetry axis. The ultimate electron donor, P700 (a pair of chlorophylls), and the tertiary acceptor, FX (a Fe4S 4 cluster), are both located on this axis, while each of the two branches is made up of a pair of chlorophylls (ec2 and ec3) and a phylloquinone (PhQ). Based on the observed biphasic reduction of FX, it has been suggested that both branches in PSI are competent for electron transfer (ET), but the nature and rate of the initial electron transfer steps have not been established. We report an ultrafast transient absorption study of Chlamydomonas reinhardtii mutants in which specific amino acids donating H-bonds to the 131-keto oxygen of either ec3A (PsaA-Tyr696) or ec3 B (PsaB-Tyr676) are converted to Phe, thus breaking the H-bond to a specific ec3 cofactor. We find that the rate of primary charge separation (CS) is lowered in both mutants, providing direct evidence that the primary ET event can be initiated independently in each branch. Furthermore, the data provide further support for the previously published model in which the initial CS event occurs within an ec2/ec3 pair, generating a primary ec2+ec3 - radical pair, followed by rapid reduction by P700 in the second ET step. A unique kinetic modeling approach allows estimation of the individual ET rates within the two cofactor branches.

    Original languageEnglish (US)
    Pages (from-to)4123-4128
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume107
    Issue number9
    DOIs
    StatePublished - Mar 2 2010

    Fingerprint

    Photosystem I Protein Complex
    Electrons
    Chlorophyll
    Vitamin K 1
    Chlamydomonas reinhardtii
    Oxygen
    Amino Acids
    Proteins

    Keywords

    • Chlamydomonas
    • Electron transfer directionality
    • Femtosecond absorption
    • Photosystem I
    • Ultrafast spectroscopy

    ASJC Scopus subject areas

    • General

    Cite this

    Independent initiation of primary electron transfer in the two branches of the photosystem I reaction center. / Müller, Marc G.; Slavov, Chavdar; Luthra, Rajiv; Redding, Kevin; Holzwarth, Alfred R.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 9, 02.03.2010, p. 4123-4128.

    Research output: Contribution to journalArticle

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    AU - Müller, Marc G.

    AU - Slavov, Chavdar

    AU - Luthra, Rajiv

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    AU - Holzwarth, Alfred R.

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