Increased synthesis of photosystem II in triticum vulgare when grown in the presence of BAS 13-338

Salil Bose, R. Mannar Mannan, C. J. Arntzen

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Addition of BAS 13-338 (4-chloro-5-dim ethylam ino-2-phenyl-3(2H)-pyridazinone) to a suspension of chloroplast thylakoids caused an increase in the I level of chlorophyll fluorescence induction without affecting the F0 level and with a slight decrease in the Fmax level in a manner similar to the addition of DCMU to a thylakoid suspension. A ddition of BAS 13-338 also inhibited the rate of Hill reaction H2O → dichlorophenol indophenol with 50% inhibition occurring at about 10 µm BAS 13-338. The inhibition was not reversed by diphenyl carbazide used as an artificial electron donor to photosystem II. These results suggest that the site of inhibition by BAS 13-338 is between Q (next to the primary electron acceptor) and plastoquinone. When the plants were grown in the presence of sublethal dose of BAS 13-338, the following changes were noted in the thylakoids of the treated plants as com pared to the thylakoids isolated from the control plants: The F0 and the norm alized variable fluorescence (ΔF/F0) levels increased, chlorophyll a/ b ratio decreased, chlorophyll/P700 ratio increased. Furthermore, the rate of photosystem II electron transport both under saturated intensity and the limiting intensity of illumination increased, and the ratio of plastoquinone to Q decreased. These observations have been interpreted as due to an increase in the ratio of photosystem II to photosystem I inplants grown in the presence of BAS 13-338.

Original languageEnglish (US)
Pages (from-to)510-514
Number of pages5
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume39
Issue number5
DOIs
StatePublished - May 1 1984

Keywords

  • BAS 13-338
  • Chlorophyll Fluorescence
  • Electron Transport
  • Photosynthesis
  • Photosystem II

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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