Increased mRNA accumulation in a psaB frame-shift mutant of Chlamydomonas reinhardtii suggests a role for translation in psaB mRNA stability

Ruohui Xu, Scott E. Bingham, Andrew Webber

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Regulation of mRNA stability is an important control in the differential accumulation of chloroplast mRNAs that occurs in response to developmental and environmental signals. The mechanism by which differential mRNA accumulation is achieved is unknown. We have examined mRNA accumulation in a chloroplast mutant of Chlamydomonas reinhardtii previously shown to contain a single AT base-pair deletion in the psaB gene. In this mutant, steady-state levels of mRNA from psaB accumulate to a level more than twice that found in cells that have had the mutation repaired by chloroplast transformation. In vivo pulse labeling of RNA shows that increased mRNA accumulation is due to a more stable transcript. We show that inhibitors of chloroplast protein synthesis also increase mRNA accumulation from the psaB gene. The results are consistent with a link between polysome association, active synthesis and stability of psaB transcripts.

Original languageEnglish (US)
Pages (from-to)465-474
Number of pages10
JournalPlant Molecular Biology
Volume22
Issue number3
DOIs
StatePublished - Jun 1993

Fingerprint

Chlamydomonas reinhardtii
RNA Stability
translation (genetics)
chloroplasts
mutants
Messenger RNA
Chloroplasts
polyribosomes
gene deletion
Chloroplast Proteins
Genes
Protein Synthesis Inhibitors
Polyribosomes
protein synthesis
RNA
mutation
Base Pairing
synthesis
Labeling
Association reactions

Keywords

  • chloroplast
  • mRNA stability
  • photosystem I
  • translation

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Increased mRNA accumulation in a psaB frame-shift mutant of Chlamydomonas reinhardtii suggests a role for translation in psaB mRNA stability. / Xu, Ruohui; Bingham, Scott E.; Webber, Andrew.

In: Plant Molecular Biology, Vol. 22, No. 3, 06.1993, p. 465-474.

Research output: Contribution to journalArticle

@article{3fffb8a55ab64804ad558e27ada84136,
title = "Increased mRNA accumulation in a psaB frame-shift mutant of Chlamydomonas reinhardtii suggests a role for translation in psaB mRNA stability",
abstract = "Regulation of mRNA stability is an important control in the differential accumulation of chloroplast mRNAs that occurs in response to developmental and environmental signals. The mechanism by which differential mRNA accumulation is achieved is unknown. We have examined mRNA accumulation in a chloroplast mutant of Chlamydomonas reinhardtii previously shown to contain a single AT base-pair deletion in the psaB gene. In this mutant, steady-state levels of mRNA from psaB accumulate to a level more than twice that found in cells that have had the mutation repaired by chloroplast transformation. In vivo pulse labeling of RNA shows that increased mRNA accumulation is due to a more stable transcript. We show that inhibitors of chloroplast protein synthesis also increase mRNA accumulation from the psaB gene. The results are consistent with a link between polysome association, active synthesis and stability of psaB transcripts.",
keywords = "chloroplast, mRNA stability, photosystem I, translation",
author = "Ruohui Xu and Bingham, {Scott E.} and Andrew Webber",
year = "1993",
month = "6",
doi = "10.1007/BF00015976",
language = "English (US)",
volume = "22",
pages = "465--474",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Increased mRNA accumulation in a psaB frame-shift mutant of Chlamydomonas reinhardtii suggests a role for translation in psaB mRNA stability

AU - Xu, Ruohui

AU - Bingham, Scott E.

AU - Webber, Andrew

PY - 1993/6

Y1 - 1993/6

N2 - Regulation of mRNA stability is an important control in the differential accumulation of chloroplast mRNAs that occurs in response to developmental and environmental signals. The mechanism by which differential mRNA accumulation is achieved is unknown. We have examined mRNA accumulation in a chloroplast mutant of Chlamydomonas reinhardtii previously shown to contain a single AT base-pair deletion in the psaB gene. In this mutant, steady-state levels of mRNA from psaB accumulate to a level more than twice that found in cells that have had the mutation repaired by chloroplast transformation. In vivo pulse labeling of RNA shows that increased mRNA accumulation is due to a more stable transcript. We show that inhibitors of chloroplast protein synthesis also increase mRNA accumulation from the psaB gene. The results are consistent with a link between polysome association, active synthesis and stability of psaB transcripts.

AB - Regulation of mRNA stability is an important control in the differential accumulation of chloroplast mRNAs that occurs in response to developmental and environmental signals. The mechanism by which differential mRNA accumulation is achieved is unknown. We have examined mRNA accumulation in a chloroplast mutant of Chlamydomonas reinhardtii previously shown to contain a single AT base-pair deletion in the psaB gene. In this mutant, steady-state levels of mRNA from psaB accumulate to a level more than twice that found in cells that have had the mutation repaired by chloroplast transformation. In vivo pulse labeling of RNA shows that increased mRNA accumulation is due to a more stable transcript. We show that inhibitors of chloroplast protein synthesis also increase mRNA accumulation from the psaB gene. The results are consistent with a link between polysome association, active synthesis and stability of psaB transcripts.

KW - chloroplast

KW - mRNA stability

KW - photosystem I

KW - translation

UR - http://www.scopus.com/inward/record.url?scp=0027619388&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027619388&partnerID=8YFLogxK

U2 - 10.1007/BF00015976

DO - 10.1007/BF00015976

M3 - Article

C2 - 8329685

AN - SCOPUS:0027619388

VL - 22

SP - 465

EP - 474

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 3

ER -