Increased insulin receptor signaling and glycogen synthase activity contribute to the synergistic effect of exercise on insulin action

Christine Y. Christ-Roberts, Thongchai Pratipanawatr, Wilailak Pratipanawatr, Rachele Berria, Renata Belfort, Lawrence J. Mandarino

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The purpose of this study was to determine the factors contributing to the ability of exercise to enhance insulin-stimulated glucose disposal. Sixteen insulin-resistant nondiabetic and seven Type 2 diabetic subjects underwent two hyperinsulinemic (40 mU·m-2·min-1) clamps, once without and once with concomitant exercise at 70% peak 02 consumption. Exercise was begun at the start of insulin infusion and was performed for 30 min. Biopsies of the vastus lateralis were performed before and after 30 min of insulin infusion (immediately after cessation of exercise). Exercise synergistically increased insulin-stimulated glucose disposal in nondiabetic [from 4.6 ± 0.4 to 9.5 ± 0.8 mg·kg fat-free mass (FFM) -1·min-1] and diabetic subjects (from 4.3 ± 1.0 to 7.9 ± 0.7 mg′kg FFM-1·min-1) subjects. The rate of glucose disposal also was significantly greater in each group after cessation of exercise. Exercise enhanced insulin-stimulated increases in glycogen synthase fractional velocity in control (from 0.07 ± 0.02 to 0.22 ± 0.05, P < 0.05) and diabetic (from 0.08 ± 0.03 to 0.15 ± 0.03, P < 0.01) subjects. Exercise also enhanced insulin-stimulated glucose storage (glycogen synthesis) in nondiabetic (2.9 ± 0.9 vs. 4.9 ± 1.1 mg·kg FFM-1· min-1) and diabetic (1.7 ± 0.5 vs. 4.2 ± 0.8 mg·kg FFM-1·min-1) subjects. Increased glucose storage accounted for the increase in whole body glucose disposal when exercise was performed during insulin stimulation in both groups; effects of exercise were correlated with enhancement of glucose disposal and glucose storage (r = 0.93, P > 0.001). Exercise synergistically enhanced insulin-stimulated insulin receptor substrate 1-associated phosphatidylinositol 3-kinase activity (P < 0.05) and Akt Ser473 phosphorylation (P < 0.05) in nondiabetic subjects but had little effect in diabetic subjects. The data indicate that exercise, performed in conjunction with insulin infusion, synergistically increases insulin-stimulated glucose disposal compared with insulin alone. In nondiabetic and diabetic subjects, increased glycogen synthase activation is likely to be involved, in part, in this effect. In nondiabetic, but not diabetic, subjects, exercise-induced enhancement of insulin stimulation of the phosphatidylinositol 3-kinase pathway is also likely to be involved in the exercise-induced synergistic enhancement of glucose disposal.

Original languageEnglish (US)
Pages (from-to)2519-2529
Number of pages11
JournalJournal of Applied Physiology
Volume95
Issue number6
StatePublished - Dec 2003
Externally publishedYes

Fingerprint

Glycogen Synthase
Insulin Receptor
Insulin
Glucose
Phosphatidylinositol 3-Kinase
Fats
Insulin Receptor Substrate Proteins
Quadriceps Muscle
Phosphorylation

Keywords

  • Akt
  • Phosphatidylinositol 3-kinase

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Christ-Roberts, C. Y., Pratipanawatr, T., Pratipanawatr, W., Berria, R., Belfort, R., & Mandarino, L. J. (2003). Increased insulin receptor signaling and glycogen synthase activity contribute to the synergistic effect of exercise on insulin action. Journal of Applied Physiology, 95(6), 2519-2529.

Increased insulin receptor signaling and glycogen synthase activity contribute to the synergistic effect of exercise on insulin action. / Christ-Roberts, Christine Y.; Pratipanawatr, Thongchai; Pratipanawatr, Wilailak; Berria, Rachele; Belfort, Renata; Mandarino, Lawrence J.

In: Journal of Applied Physiology, Vol. 95, No. 6, 12.2003, p. 2519-2529.

Research output: Contribution to journalArticle

Christ-Roberts, CY, Pratipanawatr, T, Pratipanawatr, W, Berria, R, Belfort, R & Mandarino, LJ 2003, 'Increased insulin receptor signaling and glycogen synthase activity contribute to the synergistic effect of exercise on insulin action', Journal of Applied Physiology, vol. 95, no. 6, pp. 2519-2529.
Christ-Roberts CY, Pratipanawatr T, Pratipanawatr W, Berria R, Belfort R, Mandarino LJ. Increased insulin receptor signaling and glycogen synthase activity contribute to the synergistic effect of exercise on insulin action. Journal of Applied Physiology. 2003 Dec;95(6):2519-2529.
Christ-Roberts, Christine Y. ; Pratipanawatr, Thongchai ; Pratipanawatr, Wilailak ; Berria, Rachele ; Belfort, Renata ; Mandarino, Lawrence J. / Increased insulin receptor signaling and glycogen synthase activity contribute to the synergistic effect of exercise on insulin action. In: Journal of Applied Physiology. 2003 ; Vol. 95, No. 6. pp. 2519-2529.
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