Increased furfural tolerance due to overexpression of NADH-dependent oxidoreductase FucO in Escherichia coli strains engineered for the production of ethanol and lactate

Xuan Wang, E. N. Miller, L. P. Yomano, X. Zhang, K. T. Shanmugam, L. O. Ingram

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Furfural is an important fermentation inhibitor in hemicellulose sugar syrups derived from woody biomass. The metabolism of furfural by NADPH-dependent oxidoreductases, such as YqhD (low K m for NADPH), is proposed to inhibit the growth and fermentation of xylose in Escherichia coli by competing with biosynthesis for NADPH. The discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural provided a new approach to improve furfural tolerance. Strains that produced ethanol or lactate efficiently as primary products from xylose were developed. These strains included chromosomal mutations in yqhD expression that permitted the fermentation of xylose broths containing up to 10 mM furfural. Expression of fucO from plasmids was shown to increase furfural tolerance by 50% and to permit the fermentation of 15 mM furfural. Product yields with 15 mM furfural were equivalent to those of control strains without added furfural (85% to 90% of the theoretical maximum). These two defined genetic traits can be readily transferred to enteric biocatalysts designed to produce other products. A similar strategy that minimizes the depletion of NADPH pools by native detoxification enzymes may be generally useful for other inhibitory compounds in lignocellulosic sugar streams and with other organisms.

Original languageEnglish (US)
Pages (from-to)5132-5140
Number of pages9
JournalApplied and Environmental Microbiology
Volume77
Issue number15
DOIs
StatePublished - Aug 2011
Externally publishedYes

Fingerprint

Furaldehyde
furfural
NAD (coenzyme)
oxidoreductases
ethanol production
NAD
fermentation
lactates
ethanol
Lactic Acid
Oxidoreductases
Ethanol
tolerance
Escherichia coli
NADP
NADP (coenzyme)
sugar
Fermentation
Xylose
xylose

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Increased furfural tolerance due to overexpression of NADH-dependent oxidoreductase FucO in Escherichia coli strains engineered for the production of ethanol and lactate. / Wang, Xuan; Miller, E. N.; Yomano, L. P.; Zhang, X.; Shanmugam, K. T.; Ingram, L. O.

In: Applied and Environmental Microbiology, Vol. 77, No. 15, 08.2011, p. 5132-5140.

Research output: Contribution to journalArticle

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