Increased collagen content in insulin-resistant skeletal muscle

Rachele Berria, Lishan Wang, Dawn K. Richardson, Jean Finlayson, Renata Belfort, Thongchai Pratipanawatr, Elena A. De Filippis, Sangeeta Kashyap, Lawrence J. Mandarino

    Research output: Contribution to journalArticle

    70 Citations (Scopus)

    Abstract

    Oversupply and underutilization of lipid fuels are widely recognized to be strongly associated with insulin resistance in skeletal muscle. Recent attention has focused on the mechanisms underlying this effect, and defects in mitochondrial function have emerged as a potential player in this scheme. Because evidence indicates that lipid oversupply can produce abnormalities in extracellular matrix composition and matrix changes can affect the function of mitochondria, the present study was undertaken to determine whether muscle from insulin-resistant, nondiabetic obese subjects and patients with type 2 diabetes mellitus had increased collagen content. Compared with lean control subjects, obese and type 2 diabetic subjects had reduced muscle glucose uptake (P < 0.01) and decreased insulin stimulation of tyrosine phosphorylation of insulin receptor substrate-1 and its ability to associate with phosphatidylinositol 3-kinase (P < 0.01 and P < 0.05). Because it was assayed by total hydroxyproline content, collagen abundance was increased in muscle from not only type 2 diabetic patients but also nondiabetic obese subjects (0.26 ± 0.05, 0.57 ± 0.18, and 0.67 ± 0.20 μg/mg muscle wet wt, lean controls, obese nondiabetics, and type 2 diabetics, respectively), indicating that hyperglycemia itself could not be responsible for this effect. Immunofluorescence staining of muscle biopsies indicated that there was increased abundance of types I and III collagen. We conclude that changes in the composition of the extracellular matrix are a general characteristic of insulin-resistant muscle.

    Original languageEnglish (US)
    JournalAmerican Journal of Physiology - Endocrinology and Metabolism
    Volume290
    Issue number3
    DOIs
    StatePublished - Mar 2006

    Fingerprint

    Muscle
    Skeletal Muscle
    Collagen
    Insulin
    Muscles
    Extracellular Matrix
    Phosphatidylinositol 3-Kinase
    Lipids
    Insulin Receptor Substrate Proteins
    Collagen Type III
    Hydroxyproline
    Phosphorylation
    Mitochondria
    Collagen Type I
    Biopsy
    Hyperglycemia
    Type 2 Diabetes Mellitus
    Fluorescent Antibody Technique
    Medical problems
    Tyrosine

    Keywords

    • Extracellular matrix
    • Insulin resistance
    • Type 2 diabetes mellitus

    ASJC Scopus subject areas

    • Physiology
    • Endocrinology
    • Biochemistry

    Cite this

    Berria, R., Wang, L., Richardson, D. K., Finlayson, J., Belfort, R., Pratipanawatr, T., ... Mandarino, L. J. (2006). Increased collagen content in insulin-resistant skeletal muscle. American Journal of Physiology - Endocrinology and Metabolism, 290(3). https://doi.org/10.1152/ajpendo.00202.2005

    Increased collagen content in insulin-resistant skeletal muscle. / Berria, Rachele; Wang, Lishan; Richardson, Dawn K.; Finlayson, Jean; Belfort, Renata; Pratipanawatr, Thongchai; De Filippis, Elena A.; Kashyap, Sangeeta; Mandarino, Lawrence J.

    In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 290, No. 3, 03.2006.

    Research output: Contribution to journalArticle

    Berria, R, Wang, L, Richardson, DK, Finlayson, J, Belfort, R, Pratipanawatr, T, De Filippis, EA, Kashyap, S & Mandarino, LJ 2006, 'Increased collagen content in insulin-resistant skeletal muscle', American Journal of Physiology - Endocrinology and Metabolism, vol. 290, no. 3. https://doi.org/10.1152/ajpendo.00202.2005
    Berria, Rachele ; Wang, Lishan ; Richardson, Dawn K. ; Finlayson, Jean ; Belfort, Renata ; Pratipanawatr, Thongchai ; De Filippis, Elena A. ; Kashyap, Sangeeta ; Mandarino, Lawrence J. / Increased collagen content in insulin-resistant skeletal muscle. In: American Journal of Physiology - Endocrinology and Metabolism. 2006 ; Vol. 290, No. 3.
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