Incorporation of lipid vesicles by mammalian cells provides a potential method for modifying cell behaviour

D. Papahadjopoulos; E. Mayhew; G. Poste; S. Smith; W. J. Vail

doi:10.1038/252163a0

Incorporation of lipid vesicles by mammalian cells provides a potential method for modifying cell behaviour

D. Papahadjopoulos, E. Mayhew, G. Poste, S. Smith, W. J. Vail

Research output: Contribution to journal › Article › peer-review

123 Scopus citations

Abstract

PHOSPHOLIPID vesicles (liposomes¹) have been used extensively as models for natural membranes^1,2. Multilamellar vesicles are incorporated by mammalian cells both in vivo^3,4 and in vitro ⁵. Sonication of such vesicles creates small (250-500 Å diameter) closed spherical vesicles composed of a single lipid bilayer enclosing an aqueous space². Cultured mammalian cells will also incorporate these unilamellar vesicles without cytotoxic effects⁶. We present here data on the uptake of unilamellar vesicles by cells in vitro and the use of these vesicles as carriers to introduce biologically-active molecules into specific regions of the cell.

Original language	English (US)
Pages (from-to)	163-166
Number of pages	4
Journal	Nature
Volume	252
Issue number	5479
DOIs	https://doi.org/10.1038/252163a0
State	Published - 1974
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1038/252163a0

Cite this

@article{050113ada85340e39a73c123423500d2,

title = "Incorporation of lipid vesicles by mammalian cells provides a potential method for modifying cell behaviour",

abstract = "PHOSPHOLIPID vesicles (liposomes1) have been used extensively as models for natural membranes1,2. Multilamellar vesicles are incorporated by mammalian cells both in vivo3,4 and in vitro 5. Sonication of such vesicles creates small (250-500 {\AA} diameter) closed spherical vesicles composed of a single lipid bilayer enclosing an aqueous space2. Cultured mammalian cells will also incorporate these unilamellar vesicles without cytotoxic effects6. We present here data on the uptake of unilamellar vesicles by cells in vitro and the use of these vesicles as carriers to introduce biologically-active molecules into specific regions of the cell.",

author = "D. Papahadjopoulos and E. Mayhew and G. Poste and S. Smith and Vail, {W. J.}",

year = "1974",

doi = "10.1038/252163a0",

language = "English (US)",

volume = "252",

pages = "163--166",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

number = "5479",

}

TY - JOUR

T1 - Incorporation of lipid vesicles by mammalian cells provides a potential method for modifying cell behaviour

AU - Papahadjopoulos, D.

AU - Mayhew, E.

AU - Poste, G.

AU - Smith, S.

AU - Vail, W. J.

PY - 1974

Y1 - 1974

N2 - PHOSPHOLIPID vesicles (liposomes1) have been used extensively as models for natural membranes1,2. Multilamellar vesicles are incorporated by mammalian cells both in vivo3,4 and in vitro 5. Sonication of such vesicles creates small (250-500 Å diameter) closed spherical vesicles composed of a single lipid bilayer enclosing an aqueous space2. Cultured mammalian cells will also incorporate these unilamellar vesicles without cytotoxic effects6. We present here data on the uptake of unilamellar vesicles by cells in vitro and the use of these vesicles as carriers to introduce biologically-active molecules into specific regions of the cell.

AB - PHOSPHOLIPID vesicles (liposomes1) have been used extensively as models for natural membranes1,2. Multilamellar vesicles are incorporated by mammalian cells both in vivo3,4 and in vitro 5. Sonication of such vesicles creates small (250-500 Å diameter) closed spherical vesicles composed of a single lipid bilayer enclosing an aqueous space2. Cultured mammalian cells will also incorporate these unilamellar vesicles without cytotoxic effects6. We present here data on the uptake of unilamellar vesicles by cells in vitro and the use of these vesicles as carriers to introduce biologically-active molecules into specific regions of the cell.

UR - http://www.scopus.com/inward/record.url?scp=0016309080&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016309080&partnerID=8YFLogxK

U2 - 10.1038/252163a0

DO - 10.1038/252163a0

M3 - Article

C2 - 4371572

AN - SCOPUS:0016309080

SN - 0028-0836

VL - 252

SP - 163

EP - 166

JO - Nature

JF - Nature

IS - 5479

ER -

Incorporation of lipid vesicles by mammalian cells provides a potential method for modifying cell behaviour

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this