Inactivation of viruses with a femtosecond laser via impulsive stimulated Raman scattering

Kong-Thon Tsen; Shaw Wei D Tsen; Chih Long Chang; Chien Fu Hung; T. C. Wu; B. Ramakrishna; K. Mossman; Juliann G. Kiang

doi:10.1117/12.762324

Inactivation of viruses with a femtosecond laser via impulsive stimulated Raman scattering

Kong-Thon Tsen, Shaw Wei D Tsen, Chih Long Chang, Chien Fu Hung, T. C. Wu, B. Ramakrishna, K. Mossman, Juliann G. Kiang

Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

10 Scopus citations

Abstract

The inactivation of viruses such as M13 bateriophages subject to excitations by a very low power visible femtosecond laser has been studied. Our experimental results show that for a visible femtosecond laser having λ = 425nm and a pulse width of 100 fs, the M13 bacteriophages are inactivated when the laser power density is greater than or equal to 50 MW / cm². The functionality of M13 bacteriophages has been shown to be critically dependent on the pulse width as well as power density of the excitation laser. Our work demonstrates that by using a very low power visible femtosecond laser, it is plausible to inactivate viruses such as the M13 bacteriophages through Impulsive Stimulated Raman Scattering (ISRS) process. These experimental findings lay down the foundation for a novel new avenue of selectively inactivating microorganisms while leaving the sensitive materials unharmed by manipulating and controlling with femtosecond laser systems.

Original language	English (US)
Title of host publication	Optical Interactions with Tissue and Cells XIX
DOIs	https://doi.org/10.1117/12.762324
State	Published - 2008
Event	Optical Interactions with Tissue and Cells XIX - San Jose, CA, United States Duration: Jan 21 2008 → Jan 23 2008

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6854
ISSN (Print)	1605-7422

Other

Other	Optical Interactions with Tissue and Cells XIX
Country/Territory	United States
City	San Jose, CA
Period	1/21/08 → 1/23/08

Keywords

Femtosecond laser
Impulsive stimulated Raman scattering
Inactivation
M13 phage

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.762324

Cite this

Tsen, K.-T., Tsen, S. W. D., Chang, C. L., Hung, C. F., Wu, T. C., Ramakrishna, B., Mossman, K., & Kiang, J. G. (2008). Inactivation of viruses with a femtosecond laser via impulsive stimulated Raman scattering. In Optical Interactions with Tissue and Cells XIX Article 68540N (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6854). https://doi.org/10.1117/12.762324

Inactivation of viruses with a femtosecond laser via impulsive stimulated Raman scattering. / Tsen, Kong-Thon; Tsen, Shaw Wei D; Chang, Chih Long et al.
Optical Interactions with Tissue and Cells XIX. 2008. 68540N (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6854).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Tsen, K-T, Tsen, SWD, Chang, CL, Hung, CF, Wu, TC, Ramakrishna, B, Mossman, K & Kiang, JG 2008, Inactivation of viruses with a femtosecond laser via impulsive stimulated Raman scattering. in Optical Interactions with Tissue and Cells XIX., 68540N, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6854, Optical Interactions with Tissue and Cells XIX, San Jose, CA, United States, 1/21/08. https://doi.org/10.1117/12.762324

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title = "Inactivation of viruses with a femtosecond laser via impulsive stimulated Raman scattering",

abstract = "The inactivation of viruses such as M13 bateriophages subject to excitations by a very low power visible femtosecond laser has been studied. Our experimental results show that for a visible femtosecond laser having λ = 425nm and a pulse width of 100 fs, the M13 bacteriophages are inactivated when the laser power density is greater than or equal to 50 MW / cm2. The functionality of M13 bacteriophages has been shown to be critically dependent on the pulse width as well as power density of the excitation laser. Our work demonstrates that by using a very low power visible femtosecond laser, it is plausible to inactivate viruses such as the M13 bacteriophages through Impulsive Stimulated Raman Scattering (ISRS) process. These experimental findings lay down the foundation for a novel new avenue of selectively inactivating microorganisms while leaving the sensitive materials unharmed by manipulating and controlling with femtosecond laser systems.",

keywords = "Femtosecond laser, Impulsive stimulated Raman scattering, Inactivation, M13 phage",

author = "Kong-Thon Tsen and Tsen, {Shaw Wei D} and Chang, {Chih Long} and Hung, {Chien Fu} and Wu, {T. C.} and B. Ramakrishna and K. Mossman and Kiang, {Juliann G.}",

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AU - Tsen, Kong-Thon

AU - Tsen, Shaw Wei D

AU - Chang, Chih Long

AU - Hung, Chien Fu

AU - Wu, T. C.

AU - Ramakrishna, B.

AU - Mossman, K.

AU - Kiang, Juliann G.

PY - 2008

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N2 - The inactivation of viruses such as M13 bateriophages subject to excitations by a very low power visible femtosecond laser has been studied. Our experimental results show that for a visible femtosecond laser having λ = 425nm and a pulse width of 100 fs, the M13 bacteriophages are inactivated when the laser power density is greater than or equal to 50 MW / cm2. The functionality of M13 bacteriophages has been shown to be critically dependent on the pulse width as well as power density of the excitation laser. Our work demonstrates that by using a very low power visible femtosecond laser, it is plausible to inactivate viruses such as the M13 bacteriophages through Impulsive Stimulated Raman Scattering (ISRS) process. These experimental findings lay down the foundation for a novel new avenue of selectively inactivating microorganisms while leaving the sensitive materials unharmed by manipulating and controlling with femtosecond laser systems.

AB - The inactivation of viruses such as M13 bateriophages subject to excitations by a very low power visible femtosecond laser has been studied. Our experimental results show that for a visible femtosecond laser having λ = 425nm and a pulse width of 100 fs, the M13 bacteriophages are inactivated when the laser power density is greater than or equal to 50 MW / cm2. The functionality of M13 bacteriophages has been shown to be critically dependent on the pulse width as well as power density of the excitation laser. Our work demonstrates that by using a very low power visible femtosecond laser, it is plausible to inactivate viruses such as the M13 bacteriophages through Impulsive Stimulated Raman Scattering (ISRS) process. These experimental findings lay down the foundation for a novel new avenue of selectively inactivating microorganisms while leaving the sensitive materials unharmed by manipulating and controlling with femtosecond laser systems.

KW - Femtosecond laser

KW - Impulsive stimulated Raman scattering

KW - Inactivation

KW - M13 phage

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ER -

Inactivation of viruses with a femtosecond laser via impulsive stimulated Raman scattering

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