Abstract
The artificial inhibition of expression of genes in Saccharomyces cerevisiae is not a widespread, useful phenomenon. The external guide sequence (EGS) technology, which is well-proven in bacteria and mammalian cells in tissue culture and in mice, can also be utilized in yeast. The TOP2 and SRG1 genes can be inhibited by ∼30% with EGSs in vivo. Results in vitro also show convenient cleavage of the relevant transcripts by RNase P and appropriate EGSs. The feasible constructs shown to date have an EGS covalently linked to M1 RNA, the RNA subunit of RNase P from Escherichia coli. Greater efficiency in cleavage of transcripts can be fashioned using more than one EGS targeted to different sites in a transcript and stronger promoters controlling the EGS constructs. Published by Cold Spring Harbor Laboratory Press.
Original language | English (US) |
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Pages (from-to) | 544-549 |
Number of pages | 6 |
Journal | RNA |
Volume | 17 |
Issue number | 3 |
DOIs | |
State | Published - Mar 2011 |
Externally published | Yes |
Keywords
- RNase P
- SRG1 gene
- TOP2 gene
- Target RNA
ASJC Scopus subject areas
- Molecular Biology