In vivo evaluation of injectable thermosensitive polymer with time-dependent LCST

Eric Henderson; Bae Hoon Lee; Zhanwu Cui; Ryan McLemore; Tedd A. Brandon; Brent Vernon

doi:10.1002/jbm.a.32179

In vivo evaluation of injectable thermosensitive polymer with time-dependent LCST

Eric Henderson, Bae Hoon Lee, Zhanwu Cui, Ryan McLemore, Tedd A. Brandon, Brent Vernon

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The focus of this study was to examine the biocompatibility, time-dependent LCST, and bioerodable properties of a copolymer system composed of NIPAAm, dimethyl-γ-butyrolactone (DMBL), and acrylic acid (AAc). Sprague Dawley rats were subcutaneously injected with 25 wt % solutions of poly(NIPAAm-co-DMBL- co-AAc). At predetermined times, animals were sacrificed and polymer implants were recovered for characterization via ¹H-NMR. In addition, polymer-contacting tissue sections were harvested and processed for histology. The biocompatibility of the implants was assessed by counting the number of fibroblasts and leukocytes present at the tissue-implant interface. The LCST data obtained from the in vivo implants was shown to agree with that of in vitro findings. Implant mass was shown to decrease after 4 days, indicating accelerated diffusion rates with increased implant swelling, hydrolytic degradation was confirmed with ¹H-NMR measurements. The cellular presence at the copolymer implant-tissue interface was shown to return to that of normal tissue 30 days postimplantation, which suggests a normal wound healing response.

Original language	English (US)
Pages (from-to)	1186-1197
Number of pages	12
Journal	Journal of Biomedical Materials Research - Part A
Volume	90
Issue number	4
DOIs	https://doi.org/10.1002/jbm.a.32179
State	Published - Sep 15 2009

Keywords

Bioresorption
Hydrogel
Hydrolysis
Thermally responsive material

ASJC Scopus subject areas

Ceramics and Composites
Biomaterials
Biomedical Engineering
Metals and Alloys

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abstract = "The focus of this study was to examine the biocompatibility, time-dependent LCST, and bioerodable properties of a copolymer system composed of NIPAAm, dimethyl-γ-butyrolactone (DMBL), and acrylic acid (AAc). Sprague Dawley rats were subcutaneously injected with 25 wt % solutions of poly(NIPAAm-co-DMBL- co-AAc). At predetermined times, animals were sacrificed and polymer implants were recovered for characterization via 1H-NMR. In addition, polymer-contacting tissue sections were harvested and processed for histology. The biocompatibility of the implants was assessed by counting the number of fibroblasts and leukocytes present at the tissue-implant interface. The LCST data obtained from the in vivo implants was shown to agree with that of in vitro findings. Implant mass was shown to decrease after 4 days, indicating accelerated diffusion rates with increased implant swelling, hydrolytic degradation was confirmed with 1H-NMR measurements. The cellular presence at the copolymer implant-tissue interface was shown to return to that of normal tissue 30 days postimplantation, which suggests a normal wound healing response.",

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author = "Eric Henderson and Lee, {Bae Hoon} and Zhanwu Cui and Ryan McLemore and Brandon, {Tedd A.} and Brent Vernon",

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doi = "10.1002/jbm.a.32179",

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AU - Lee, Bae Hoon

AU - Cui, Zhanwu

AU - McLemore, Ryan

AU - Brandon, Tedd A.

AU - Vernon, Brent

PY - 2009/9/15

Y1 - 2009/9/15

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AB - The focus of this study was to examine the biocompatibility, time-dependent LCST, and bioerodable properties of a copolymer system composed of NIPAAm, dimethyl-γ-butyrolactone (DMBL), and acrylic acid (AAc). Sprague Dawley rats were subcutaneously injected with 25 wt % solutions of poly(NIPAAm-co-DMBL- co-AAc). At predetermined times, animals were sacrificed and polymer implants were recovered for characterization via 1H-NMR. In addition, polymer-contacting tissue sections were harvested and processed for histology. The biocompatibility of the implants was assessed by counting the number of fibroblasts and leukocytes present at the tissue-implant interface. The LCST data obtained from the in vivo implants was shown to agree with that of in vitro findings. Implant mass was shown to decrease after 4 days, indicating accelerated diffusion rates with increased implant swelling, hydrolytic degradation was confirmed with 1H-NMR measurements. The cellular presence at the copolymer implant-tissue interface was shown to return to that of normal tissue 30 days postimplantation, which suggests a normal wound healing response.

KW - Bioresorption

KW - Hydrogel

KW - Hydrolysis

KW - Thermally responsive material

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In vivo evaluation of injectable thermosensitive polymer with time-dependent LCST

Abstract

Keywords

ASJC Scopus subject areas

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