In vivo distribution and dynamics of DNA topoisomerase II in drosophila embryonic nuclei and chromosomes

Jason R. Swedlow, Neil Osheroff, Tim Karr, John W. Sedat, David A. Agard

Research output: Contribution to journalConference articlepeer-review

Abstract

DNA topoisomerase II is an ATP-dependent double-stranded DNA strand-passing enzyme that is necessary for full condensation of chromosomes and for complete segregation of sister chromatids at mitosis in vivo and in vitro. The chromosomal distribution of topoisomerase II in vivo is monitored during mitosis in the Drosophila embryo. For this experiment, the embryonic nuclei synchronously progress through 13 mitotic cycles, an ideal system to follow nuclear and chromosomal dynamics.

Original languageEnglish (US)
Pages (from-to)74-75
Number of pages2
JournalProceedings - Annual Meeting, Microscopy Society of America
StatePublished - Dec 1 1993
Externally publishedYes
EventProceedings of the 51st Annual Meeting Microscopy Society of America - Cincinnati, OH, USA
Duration: Aug 1 1993Aug 6 1993

ASJC Scopus subject areas

  • Engineering(all)

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