In vivo activation of chicken macrophages by infectious bursal disease virus

J. M. Palmquist, M. Khatri, R. M. Cha, B. M. Goddeeris, B. Walcheck, J. M. Sharma

Research output: Contribution to journalArticle

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Abstract

Infectious bursal disease virus (IBDV) infects and replicates in the dividing B lymphocytes of chickens. In the present study, the in vivo effect of IBDV infection on chicken macrophage populations and macrophage activation were examined. Specific-pathogen-free chickens were exposed to virulent IBDV and splenic macrophages were recovered during the acute phase of the disease. At 3 and 5 days post-infection (dpi), spleens of virus-exposed chickens had fewer macrophages than those of virus-free controls (p < 0.05). Confocal microscopic examination revealed cells that were positive for both KUL01 (macrophage surface marker) and R63 (IBDV-VP2), indicating presence of the virus in macrophages. MQ-NCSU cells, an avian macrophage cell line, were susceptible to replication of IBDV. In addition, splenic macrophages were activated and had temporarily increased levels of mRNA transcripts of pro-inflammatory mediators, including IL-1β, IL-6, IL-18, and iNOS. The robust expression of proinflammatory cytokine transcripts, along with a decrease in macrophage numbers, suggest that IBDV activates and may lead to a reduction of resident macrophages in vivo.

Original languageEnglish (US)
Pages (from-to)305-315
Number of pages11
JournalViral Immunology
Volume19
Issue number2
DOIs
StatePublished - 2006
Externally publishedYes

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Infectious bursal disease virus
Macrophage Activation
Chickens
Macrophages
Virus Diseases
Viruses
Specific Pathogen-Free Organisms
Interleukin-18
Acute Disease
Interleukin-1
Interleukin-6
B-Lymphocytes
Spleen
Cytokines

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Palmquist, J. M., Khatri, M., Cha, R. M., Goddeeris, B. M., Walcheck, B., & Sharma, J. M. (2006). In vivo activation of chicken macrophages by infectious bursal disease virus. Viral Immunology, 19(2), 305-315. https://doi.org/10.1089/vim.2006.19.305

In vivo activation of chicken macrophages by infectious bursal disease virus. / Palmquist, J. M.; Khatri, M.; Cha, R. M.; Goddeeris, B. M.; Walcheck, B.; Sharma, J. M.

In: Viral Immunology, Vol. 19, No. 2, 2006, p. 305-315.

Research output: Contribution to journalArticle

Palmquist, JM, Khatri, M, Cha, RM, Goddeeris, BM, Walcheck, B & Sharma, JM 2006, 'In vivo activation of chicken macrophages by infectious bursal disease virus', Viral Immunology, vol. 19, no. 2, pp. 305-315. https://doi.org/10.1089/vim.2006.19.305
Palmquist JM, Khatri M, Cha RM, Goddeeris BM, Walcheck B, Sharma JM. In vivo activation of chicken macrophages by infectious bursal disease virus. Viral Immunology. 2006;19(2):305-315. https://doi.org/10.1089/vim.2006.19.305
Palmquist, J. M. ; Khatri, M. ; Cha, R. M. ; Goddeeris, B. M. ; Walcheck, B. ; Sharma, J. M. / In vivo activation of chicken macrophages by infectious bursal disease virus. In: Viral Immunology. 2006 ; Vol. 19, No. 2. pp. 305-315.
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