In vitro suppression of T-cell mitogenic response and tumor cell proliferation by spleen macrophages from normal chickens

J. M. Sharma

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Adherent cells isolated from spleen of normal specific pathogen-free chickens inhibited mitogen-induced lastogenesis of autochthonous, syngeneic, or allogeneic lymphocytes. The adherent cells were also inhibitory to in vitro proliferation of cells of a rapidly dividing tumor line, MDCC-MSB-1, derived from a lymphoma induced by Marek's disease virus. The effector cell of suppression of both lymphoproliferative functions appeared to be a macrophage because the suppressive activity of adherent cells could be abrogated by pretreatment with carrageenan but not with antisera specific to chicken T or B cells. The proportion of macrophages needed for effective suppression was substantially higher than the porportion of macrophages ordinarily pesent in spleen of normal, unstimulated chickens. This heretofore unrecognized suppressive capability of normal, presumably resting macrophages in chickens needs to be evaluated in light of the fact that suppressor macrophages have been detected in certain infections.

Original languageEnglish (US)
Pages (from-to)914-922
Number of pages9
JournalInfection and Immunity
Volume28
Issue number3
StatePublished - 1980
Externally publishedYes

Fingerprint

Chickens
Spleen
Macrophages
Cell Proliferation
T-Lymphocytes
Neoplasms
Marek Disease
Specific Pathogen-Free Organisms
Carrageenan
Mitogens
Immune Sera
Lymphoma
B-Lymphocytes
In Vitro Techniques
Lymphocytes
Viruses
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

In vitro suppression of T-cell mitogenic response and tumor cell proliferation by spleen macrophages from normal chickens. / Sharma, J. M.

In: Infection and Immunity, Vol. 28, No. 3, 1980, p. 914-922.

Research output: Contribution to journalArticle

@article{07837b238623465a9fcb5976221fa823,
title = "In vitro suppression of T-cell mitogenic response and tumor cell proliferation by spleen macrophages from normal chickens",
abstract = "Adherent cells isolated from spleen of normal specific pathogen-free chickens inhibited mitogen-induced lastogenesis of autochthonous, syngeneic, or allogeneic lymphocytes. The adherent cells were also inhibitory to in vitro proliferation of cells of a rapidly dividing tumor line, MDCC-MSB-1, derived from a lymphoma induced by Marek's disease virus. The effector cell of suppression of both lymphoproliferative functions appeared to be a macrophage because the suppressive activity of adherent cells could be abrogated by pretreatment with carrageenan but not with antisera specific to chicken T or B cells. The proportion of macrophages needed for effective suppression was substantially higher than the porportion of macrophages ordinarily pesent in spleen of normal, unstimulated chickens. This heretofore unrecognized suppressive capability of normal, presumably resting macrophages in chickens needs to be evaluated in light of the fact that suppressor macrophages have been detected in certain infections.",
author = "Sharma, {J. M.}",
year = "1980",
language = "English (US)",
volume = "28",
pages = "914--922",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - In vitro suppression of T-cell mitogenic response and tumor cell proliferation by spleen macrophages from normal chickens

AU - Sharma, J. M.

PY - 1980

Y1 - 1980

N2 - Adherent cells isolated from spleen of normal specific pathogen-free chickens inhibited mitogen-induced lastogenesis of autochthonous, syngeneic, or allogeneic lymphocytes. The adherent cells were also inhibitory to in vitro proliferation of cells of a rapidly dividing tumor line, MDCC-MSB-1, derived from a lymphoma induced by Marek's disease virus. The effector cell of suppression of both lymphoproliferative functions appeared to be a macrophage because the suppressive activity of adherent cells could be abrogated by pretreatment with carrageenan but not with antisera specific to chicken T or B cells. The proportion of macrophages needed for effective suppression was substantially higher than the porportion of macrophages ordinarily pesent in spleen of normal, unstimulated chickens. This heretofore unrecognized suppressive capability of normal, presumably resting macrophages in chickens needs to be evaluated in light of the fact that suppressor macrophages have been detected in certain infections.

AB - Adherent cells isolated from spleen of normal specific pathogen-free chickens inhibited mitogen-induced lastogenesis of autochthonous, syngeneic, or allogeneic lymphocytes. The adherent cells were also inhibitory to in vitro proliferation of cells of a rapidly dividing tumor line, MDCC-MSB-1, derived from a lymphoma induced by Marek's disease virus. The effector cell of suppression of both lymphoproliferative functions appeared to be a macrophage because the suppressive activity of adherent cells could be abrogated by pretreatment with carrageenan but not with antisera specific to chicken T or B cells. The proportion of macrophages needed for effective suppression was substantially higher than the porportion of macrophages ordinarily pesent in spleen of normal, unstimulated chickens. This heretofore unrecognized suppressive capability of normal, presumably resting macrophages in chickens needs to be evaluated in light of the fact that suppressor macrophages have been detected in certain infections.

UR - http://www.scopus.com/inward/record.url?scp=0018901884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018901884&partnerID=8YFLogxK

M3 - Article

VL - 28

SP - 914

EP - 922

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 3

ER -