In vitro suppression as a tool for the investigation of translation initiation

Vladimir A. Karginov, Sergey V. Mamaev, Sidney Hecht

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

An in vitro protein synthesizing system that employs rabbit reticulocyte lysates has been employed for protein production from mRNAs containing nonsense (UAG) codons in the presence of misacylated suppressor tRNAs. The system includes a misacylated Escherichia coli tRNA(Ala)(CUA) that functions at least as efficiently as any suppressor tRNA transcript reported to date and which has been shown not to be a substrate for (re)activation by alanyl-tRNA synthetase. Application of the optimized system for preparation of dihydrofolate analogs has also permitted analysis of competing mechanisms that control the sites(s) of translation initiation.

Original languageEnglish (US)
Pages (from-to)3912-3916
Number of pages5
JournalNucleic Acids Research
Volume25
Issue number19
DOIs
StatePublished - Oct 1 1997
Externally publishedYes

Fingerprint

Transfer RNA
Alanine-tRNA Ligase
RNA, Transfer, Ala
Terminator Codon
Nonsense Codon
Reticulocytes
Proteins
Escherichia coli
Rabbits
Messenger RNA
In Vitro Techniques
dihydrofolate

ASJC Scopus subject areas

  • Genetics

Cite this

In vitro suppression as a tool for the investigation of translation initiation. / Karginov, Vladimir A.; Mamaev, Sergey V.; Hecht, Sidney.

In: Nucleic Acids Research, Vol. 25, No. 19, 01.10.1997, p. 3912-3916.

Research output: Contribution to journalArticle

Karginov, Vladimir A. ; Mamaev, Sergey V. ; Hecht, Sidney. / In vitro suppression as a tool for the investigation of translation initiation. In: Nucleic Acids Research. 1997 ; Vol. 25, No. 19. pp. 3912-3916.
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