In vitro suppression as a tool for the investigation of translation initiation

Vladimir A. Karginov, Sergey V. Mamaev, Sidney M. Hecht

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

An in vitro protein synthesizing system that employs rabbit reticulocyte lysates has been employed for protein production from mRNAs containing nonsense (UAG) codons in the presence of misacylated suppressor tRNAs. The system includes a misacylated Escherichia coli tRNA(Ala)(CUA) that functions at least as efficiently as any suppressor tRNA transcript reported to date and which has been shown not to be a substrate for (re)activation by alanyl-tRNA synthetase. Application of the optimized system for preparation of dihydrofolate analogs has also permitted analysis of competing mechanisms that control the sites(s) of translation initiation.

Original languageEnglish (US)
Pages (from-to)3912-3916
Number of pages5
JournalNucleic acids research
Volume25
Issue number19
DOIs
StatePublished - Oct 1 1997
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'In vitro suppression as a tool for the investigation of translation initiation'. Together they form a unique fingerprint.

  • Cite this