In vitro interaction of coronaviruses with primate and human brain microvascular endothelial cells

G. F. Cabirac, R. S. Murray, L. B. McLaughlin, D. M. Skolnick, B. Hogue, K. Dorovini- Zis, P. J. Didier

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Primary human and primate brain microvascular endothelial cells were tested for permissiveness to coronaviruses JHM and 229E. While sub-genomic viral RNAs could be detected up to 72 hours post-infection, primate cells were abortively infected and neither virus caused cytopathology. Human cells were non-permissive for JHM but permissive for 229E replication; peak production of progeny 229E and observable cytopathic effects occurred approximately 22 and 32 hour post-infection, respectively. Using the criterion of cytopathology induction in infected endothelial cells, 229E was compared to other human RNA and DNA viruses. In addition, virus induced modulation of intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1) and HLA I was monitored by immunostaining of infected cells.

Original languageEnglish (US)
Pages (from-to)79-88
Number of pages10
JournalAdvances in experimental medicine and biology
Volume380
DOIs
StatePublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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