In vitro inhibition of adherence of Streptococcus mutans strains by nonadherent mutants of S. mutans 6715

H. Murchison, S. Larrimore, R. Curtiss

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Four nonadherent mutants from Streptococcus mutans 6715 mutant UAB66 (serotype g) with similar phenotypes were shown to inhibit the adherence of adherence-proficient S. mutans serotypes c and g strains. One mutant, UAB108, was shown to inhibit adherence by wild-type strains representing serotypes a, d, and e as well. This inhibition of adherence was seen with pairs of strains grown in partially defined (PD) medium supplemented with 1% sucrose in both microtiter plates and glass tubes. The inhibiting factor was present in culture supernatant fluids of inhibiting strains grown in PD medium plus 1% sucrose and was heat stable. Ethanol precipitation of culture supernatant fluids of these strains yielded a water-soluble polymer which effectively inhibited the adherence of UAB66. This polymer, isolated from UAB108, was also shown to inhibit the adherence of UAB66 at lower concentrations than that needed to inhibit adherence with dextran T10. Partially purified glucosyltransferase, isolated from the culture supernatant fluids of glucose-grown UAB108, produced a water-soluble glucan which was shown to inhibit the adherence of UAB66 as well. The methods developed permit rapid screening for strains or mutants of strains or both that inhibit adherence or plaque formation or both by wild-type strains of S. mutans.

Original languageEnglish (US)
Pages (from-to)826-832
Number of pages7
JournalInfection and Immunity
Volume50
Issue number3
StatePublished - 1985
Externally publishedYes

Fingerprint

Streptococcus mutans
Sucrose
Polymers
Glucosyltransferases
Glucans
Water
Dextrans
Glass
Ethanol
Hot Temperature
Phenotype
Glucose
Serogroup
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

In vitro inhibition of adherence of Streptococcus mutans strains by nonadherent mutants of S. mutans 6715. / Murchison, H.; Larrimore, S.; Curtiss, R.

In: Infection and Immunity, Vol. 50, No. 3, 1985, p. 826-832.

Research output: Contribution to journalArticle

Murchison, H. ; Larrimore, S. ; Curtiss, R. / In vitro inhibition of adherence of Streptococcus mutans strains by nonadherent mutants of S. mutans 6715. In: Infection and Immunity. 1985 ; Vol. 50, No. 3. pp. 826-832.
@article{872250d166b04f0ab99cc39af2743d5c,
title = "In vitro inhibition of adherence of Streptococcus mutans strains by nonadherent mutants of S. mutans 6715",
abstract = "Four nonadherent mutants from Streptococcus mutans 6715 mutant UAB66 (serotype g) with similar phenotypes were shown to inhibit the adherence of adherence-proficient S. mutans serotypes c and g strains. One mutant, UAB108, was shown to inhibit adherence by wild-type strains representing serotypes a, d, and e as well. This inhibition of adherence was seen with pairs of strains grown in partially defined (PD) medium supplemented with 1{\%} sucrose in both microtiter plates and glass tubes. The inhibiting factor was present in culture supernatant fluids of inhibiting strains grown in PD medium plus 1{\%} sucrose and was heat stable. Ethanol precipitation of culture supernatant fluids of these strains yielded a water-soluble polymer which effectively inhibited the adherence of UAB66. This polymer, isolated from UAB108, was also shown to inhibit the adherence of UAB66 at lower concentrations than that needed to inhibit adherence with dextran T10. Partially purified glucosyltransferase, isolated from the culture supernatant fluids of glucose-grown UAB108, produced a water-soluble glucan which was shown to inhibit the adherence of UAB66 as well. The methods developed permit rapid screening for strains or mutants of strains or both that inhibit adherence or plaque formation or both by wild-type strains of S. mutans.",
author = "H. Murchison and S. Larrimore and R. Curtiss",
year = "1985",
language = "English (US)",
volume = "50",
pages = "826--832",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - In vitro inhibition of adherence of Streptococcus mutans strains by nonadherent mutants of S. mutans 6715

AU - Murchison, H.

AU - Larrimore, S.

AU - Curtiss, R.

PY - 1985

Y1 - 1985

N2 - Four nonadherent mutants from Streptococcus mutans 6715 mutant UAB66 (serotype g) with similar phenotypes were shown to inhibit the adherence of adherence-proficient S. mutans serotypes c and g strains. One mutant, UAB108, was shown to inhibit adherence by wild-type strains representing serotypes a, d, and e as well. This inhibition of adherence was seen with pairs of strains grown in partially defined (PD) medium supplemented with 1% sucrose in both microtiter plates and glass tubes. The inhibiting factor was present in culture supernatant fluids of inhibiting strains grown in PD medium plus 1% sucrose and was heat stable. Ethanol precipitation of culture supernatant fluids of these strains yielded a water-soluble polymer which effectively inhibited the adherence of UAB66. This polymer, isolated from UAB108, was also shown to inhibit the adherence of UAB66 at lower concentrations than that needed to inhibit adherence with dextran T10. Partially purified glucosyltransferase, isolated from the culture supernatant fluids of glucose-grown UAB108, produced a water-soluble glucan which was shown to inhibit the adherence of UAB66 as well. The methods developed permit rapid screening for strains or mutants of strains or both that inhibit adherence or plaque formation or both by wild-type strains of S. mutans.

AB - Four nonadherent mutants from Streptococcus mutans 6715 mutant UAB66 (serotype g) with similar phenotypes were shown to inhibit the adherence of adherence-proficient S. mutans serotypes c and g strains. One mutant, UAB108, was shown to inhibit adherence by wild-type strains representing serotypes a, d, and e as well. This inhibition of adherence was seen with pairs of strains grown in partially defined (PD) medium supplemented with 1% sucrose in both microtiter plates and glass tubes. The inhibiting factor was present in culture supernatant fluids of inhibiting strains grown in PD medium plus 1% sucrose and was heat stable. Ethanol precipitation of culture supernatant fluids of these strains yielded a water-soluble polymer which effectively inhibited the adherence of UAB66. This polymer, isolated from UAB108, was also shown to inhibit the adherence of UAB66 at lower concentrations than that needed to inhibit adherence with dextran T10. Partially purified glucosyltransferase, isolated from the culture supernatant fluids of glucose-grown UAB108, produced a water-soluble glucan which was shown to inhibit the adherence of UAB66 as well. The methods developed permit rapid screening for strains or mutants of strains or both that inhibit adherence or plaque formation or both by wild-type strains of S. mutans.

UR - http://www.scopus.com/inward/record.url?scp=0022376483&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022376483&partnerID=8YFLogxK

M3 - Article

C2 - 4066034

AN - SCOPUS:0022376483

VL - 50

SP - 826

EP - 832

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 3

ER -