In vitro growth inhibition of a broad spectrum of tumor cell lines by activated human dendritic cells

Andrei I. Chapoval, Koji Tamada, Lieping Chen

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

Dendritic cells (DCs) are critical subsets of leukocytes providing antigen presentation for initiation of humoral and cellular immune responses. Their role as effector cells in tumor resistance, however, is less known. We report here that human DCs generated by culturing plastic-adherent peripheral blood monocytes in the presence of granulocyte-monocyte colony-stimulating factor (GM-CSF) and interleukin-4 have potent growth-inhibition activity in vitro on a wide spectrum of human tumor lines of different tissue origin. Proinflammatory stimuli lipopolysaccharide (LPS) and interferon-γ, but not tumor necrosis factor-α and CD40 signaling, can further enhance DC-mediated inhibition of tumor growth. The growth inhibition requires contact between DCs and tumor cells while LPS treatment enhances the antitumor activity in DC culture supernatants. Our results suggest that in addition to their predominant role as regulatory cells, activated DCs are also potential effector cells in tumor immunity. (C) 2000 by The American Society of Hematology.

Original languageEnglish (US)
Pages (from-to)2346-2351
Number of pages6
JournalBlood
Volume95
Issue number7
DOIs
StatePublished - Apr 1 2000

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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