In vitro evidence of two-component system phosphorylation between the Mycobacterium tuberculosis TrcR/TrcS proteins

Shelley Haydel, Nancy E. Dunlap, William H. Benjamin

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Two-component regulatory proteins, histidine kinases and response regulators, function in bacteria as sensing and adaptive factors in response to a wide range of environmental stimuli. Conserved histidine and glycine regions of histidine kinase sensor proteins were used to design degenerate oligonucleotide primers for amplification of DNA fragments from Mycobacterium tuberculosis. Two adjacent genes, trcR and trcS, which encode a response regulator and a histidine kinase, respectively, have been identified. Full-length and truncated TrcR and TrcS proteins have been expressed in Escherichia coli. Difficulties in expressing recombinant full-length TrcS and a truncated N-terminal form of TrcS reveal that the transmembrane domains are toxic to E. coil. Overexpressed truncated C-terminal transmitter domains of TrcS have been autophosphorylated in vitro and have transphosphorylated both the full-length recombinant TrcR protein and the N-terminal receiver/regulator domain of TrcR. In vitro autophosphorylation of TrcS requires the presence of Mn2+ or Ca2+ as a divalent cation cofactor and subsequent transphosphorylation of TrcR is evident in the presence of TrcS-phosphate and Ca2+. Transphosphorylation between these two proteins provides evidence that these M. tuberculosis genes encode functional two-component system regulatory proteins that are members of a signal transduction circuit.

Original languageEnglish (US)
Pages (from-to)195-206
Number of pages12
JournalMicrobial Pathogenesis
Volume26
Issue number4
DOIs
StatePublished - Apr 1999
Externally publishedYes

Fingerprint

Mycobacterium tuberculosis
Phosphorylation
Proteins
DNA Primers
Poisons
Divalent Cations
Recombinant Proteins
Histidine
Glycine
Genes
Signal Transduction
Phosphates
Escherichia coli
Bacteria
DNA
In Vitro Techniques
Histidine Kinase

Keywords

  • Histidine kinase
  • Mycobacterium tuberculosis
  • Phosphorylation
  • Response regulator
  • Sensor
  • Two-component system

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

In vitro evidence of two-component system phosphorylation between the Mycobacterium tuberculosis TrcR/TrcS proteins. / Haydel, Shelley; Dunlap, Nancy E.; Benjamin, William H.

In: Microbial Pathogenesis, Vol. 26, No. 4, 04.1999, p. 195-206.

Research output: Contribution to journalArticle

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