We used the recombinant chicken interferon-γ (ChIFN-γ) to determine its in vitro effects on chicken immune cells. We found that ChIFN-γ induced nitric oxide (NO) production, upregulated Ia expression on the cell surface, and inhibited the replication of Newcastle disease virus in NCSU and HD11 cells (chicken macrophage cell lines). In addition, ChIFN-γ had an antiproliferative effect on RP9 cells, a chicken B cell line. Finally, ChIFN- γ inhibited mitogenic proliferation of normal chicken spleen cells and induced the cells to generate NO. Inhibition of viral replication and mitogenic proliferation of normal cells were correlated with NO production. We conclude that recombinant chicken ChIFN-γ modulates chicken immune cells.
ASJC Scopus subject areas
- Cell Biology