In vitro effects of recombinant chicken interferon-γ on immune cells

Hung Yueh Yeh; Barbara J. Winslow; David E. Junker; Jagdev M. Sharma

doi:10.1089/107999099313848

In vitro effects of recombinant chicken interferon-γ on immune cells

Hung Yueh Yeh, Barbara J. Winslow, David E. Junker, Jagdev M. Sharma

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

We used the recombinant chicken interferon-γ (ChIFN-γ) to determine its in vitro effects on chicken immune cells. We found that ChIFN-γ induced nitric oxide (NO) production, upregulated Ia expression on the cell surface, and inhibited the replication of Newcastle disease virus in NCSU and HD11 cells (chicken macrophage cell lines). In addition, ChIFN-γ had an antiproliferative effect on RP9 cells, a chicken B cell line. Finally, ChIFN- γ inhibited mitogenic proliferation of normal chicken spleen cells and induced the cells to generate NO. Inhibition of viral replication and mitogenic proliferation of normal cells were correlated with NO production. We conclude that recombinant chicken ChIFN-γ modulates chicken immune cells.

Original language	English (US)
Pages (from-to)	687-691
Number of pages	5
Journal	Journal of Interferon and Cytokine Research
Volume	19
Issue number	6
DOIs	https://doi.org/10.1089/107999099313848
State	Published - 1999
Externally published	Yes

ASJC Scopus subject areas

Immunology
Cell Biology
Virology

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title = "In vitro effects of recombinant chicken interferon-γ on immune cells",

abstract = "We used the recombinant chicken interferon-γ (ChIFN-γ) to determine its in vitro effects on chicken immune cells. We found that ChIFN-γ induced nitric oxide (NO) production, upregulated Ia expression on the cell surface, and inhibited the replication of Newcastle disease virus in NCSU and HD11 cells (chicken macrophage cell lines). In addition, ChIFN-γ had an antiproliferative effect on RP9 cells, a chicken B cell line. Finally, ChIFN- γ inhibited mitogenic proliferation of normal chicken spleen cells and induced the cells to generate NO. Inhibition of viral replication and mitogenic proliferation of normal cells were correlated with NO production. We conclude that recombinant chicken ChIFN-γ modulates chicken immune cells.",

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AU - Yeh, Hung Yueh

AU - Winslow, Barbara J.

AU - Junker, David E.

AU - Sharma, Jagdev M.

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Y1 - 1999

N2 - We used the recombinant chicken interferon-γ (ChIFN-γ) to determine its in vitro effects on chicken immune cells. We found that ChIFN-γ induced nitric oxide (NO) production, upregulated Ia expression on the cell surface, and inhibited the replication of Newcastle disease virus in NCSU and HD11 cells (chicken macrophage cell lines). In addition, ChIFN-γ had an antiproliferative effect on RP9 cells, a chicken B cell line. Finally, ChIFN- γ inhibited mitogenic proliferation of normal chicken spleen cells and induced the cells to generate NO. Inhibition of viral replication and mitogenic proliferation of normal cells were correlated with NO production. We conclude that recombinant chicken ChIFN-γ modulates chicken immune cells.

AB - We used the recombinant chicken interferon-γ (ChIFN-γ) to determine its in vitro effects on chicken immune cells. We found that ChIFN-γ induced nitric oxide (NO) production, upregulated Ia expression on the cell surface, and inhibited the replication of Newcastle disease virus in NCSU and HD11 cells (chicken macrophage cell lines). In addition, ChIFN-γ had an antiproliferative effect on RP9 cells, a chicken B cell line. Finally, ChIFN- γ inhibited mitogenic proliferation of normal chicken spleen cells and induced the cells to generate NO. Inhibition of viral replication and mitogenic proliferation of normal cells were correlated with NO production. We conclude that recombinant chicken ChIFN-γ modulates chicken immune cells.

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In vitro effects of recombinant chicken interferon-γ on immune cells

Abstract

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