In vitro characterization of reactive oxygen species (ROS) generation by the commercially available Mesosilver™ dietary supplement

Hongyan Rong, Shikha Garg, Paul Westerhoff, T. David Waite

Research output: Contribution to journalArticle

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Abstract

Silver nanoparticles (AgNPs) are frequently orally consumed as dietary supplements with various commercially available products (e.g., Nano-silver™ and Mesosilver™) claimed to yield health benefits across an array of areas. While the mechanism by which AgNPs might provide health benefits is unclear, these benefits could potentially be related to either the release of dissolved monovalent Ag species (such as Ag+) or the ability of AgNPs to generate reactive oxygen species (ROS) and subsequent influence on the gut microbiome or eukaryotic cells. In this study, we investigate the in vitro Ag(i) and ROS (particularly hydrogen peroxide (H2O2) and hydroxyl radicals (OH)) generation by Mesosilver™ over a range of pHs (2-7) typical of the human gut and upper digestive system. Our results show that Mesosilver™ undergoes rapid oxygen-mediated oxidative dissolution and associated H2O2 production with the generation rate of both Ag(i) and H2O2 increasing with decrease in pH and increase in NaCl concentration. No impact of increase in temperature from 22 °C to 37 °C was observed on the oxidative dissolution of Mesosilver™. Addition of H2O2 further accelerates the dissolution of Mesosilver™ and causes production of OH under all pH conditions investigated here. The presence of pepsin, an enzyme present in gastric fluid, resulted in a slight decrease in the extent of release of Ag(i) and H2O2 generation under acidic conditions. A kinetic model is developed based on our experimental results enabling prediction of the dissolved Ag(i) and ROS yield on dissolution of these nanoparticles under conditions typical of the human digestive system.

Original languageEnglish (US)
Pages (from-to)2686-2698
Number of pages13
JournalEnvironmental Science: Nano
Volume5
Issue number11
DOIs
StatePublished - Jan 1 2018

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Dietary supplements
Reactive Oxygen Species
Dissolution
Digestive system
dissolution
digestive system
Oxygen
Silver
silver
Health
Nanoparticles
Pepsin A
hydroxyl radical
Hydrogen peroxide
Hydroxyl Radical
hydrogen peroxide
Hydrogen Peroxide
Enzymes
enzyme
kinetics

ASJC Scopus subject areas

  • Materials Science (miscellaneous)
  • Environmental Science(all)

Cite this

In vitro characterization of reactive oxygen species (ROS) generation by the commercially available Mesosilver™ dietary supplement. / Rong, Hongyan; Garg, Shikha; Westerhoff, Paul; Waite, T. David.

In: Environmental Science: Nano, Vol. 5, No. 11, 01.01.2018, p. 2686-2698.

Research output: Contribution to journalArticle

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