IN SITU DETERMINATION OF KINETIC PARAMETERS FOR BIOFILMS: ISOLATION AND CHARACTERIZATION OF OLIGOTROPHIC BIOFILMS.

Bruce Rittmann, LouAnn Crawford, Cynthia K. Tuck, Eun Namkung

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

This article develops and utilizes an in situ technique to estimate the Monod half-maximum rate concentration, K//s, and the maximum specific utilization rate constant, k, for biofilms. The technique employs a curve-matching method with kinetic results from several short-term experiments with completely mixed biofilm reactors. Use of the in situ method eliminates the two drawbacks of using conventional suspended-growth measurements to characterize biofilm: possible alteration of cell physiology and a major investment to run the suspended-growth tests. Results with five cultures of biofilm-forming oligotrophs demonstrated the in situ technique and supported the hypothesis that K//s values were lower for the biofilm oligotrophs than for typical copiotrophs.

Original languageEnglish (US)
Pages (from-to)1753-1760
Number of pages8
JournalBiotechnology and Bioengineering
Volume28
Issue number11
StatePublished - Nov 1986
Externally publishedYes

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Biofilms
Kinetic parameters
Cell Physiological Phenomena
Physiology
Growth
Rate constants
Kinetics
Experiments

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

IN SITU DETERMINATION OF KINETIC PARAMETERS FOR BIOFILMS : ISOLATION AND CHARACTERIZATION OF OLIGOTROPHIC BIOFILMS. / Rittmann, Bruce; Crawford, LouAnn; Tuck, Cynthia K.; Namkung, Eun.

In: Biotechnology and Bioengineering, Vol. 28, No. 11, 11.1986, p. 1753-1760.

Research output: Contribution to journalArticle

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