Abstract
Groups of specific pathogen-free chickens aged 3 weeks were inoculated with one of three strains of infectious bursal disease virus (IBDV), namely: (1) a classical virulent strain, IM-IBDV; (2) an antigenic variant-E strain, VE-IBDV; and (3) an attenuated vaccine strain, B2-IBDV. The bursa of Fabricius and thymus were examined 1 to 5 days after inoculation for histopathological lesions, IBDV antigen, and in-situ apoptosis. Infection of chickens with IM-, VE-, or B2-IBDV resulted in the appearance of large numbers of apoptotic bursal lymphocytes, both in IBDV antigen-positive and antigen-negative bursal follicles. B2-IBDV induced a slower increase in the IBDV antigen-positive cells and apoptotic cells than did IM- and VE-IBDV. The results suggested that virulent strains and vaccine strains of IBDV enhance endogenous apoptosis of bursal lymphocytes that are free of detectable IBDV antigen. Infection of chickens with IM-IBDV, but not with VE- or B2-IBDV, caused a significant increase in apoptotic cells in the thymic cortical lymphocytes. The apoptotic lymphocytes in the thymus were negative for IBDV antigen. This result indicated that histopathological lesions in the thymus of IM-IBDV-infected chickens may be due to enhanced endogenous apoptosis of thymic lymphocytes without IBDV antigen production in the cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 15-27 |
| Number of pages | 13 |
| Journal | Journal of Comparative Pathology |
| Volume | 118 |
| Issue number | 1 |
| State | Published - 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Animal Science and Zoology
Cite this
In-situ apoptosis in chickens infected with infectious bursal disease virus. / Tanimura, N.; Sharma, J. M.
In: Journal of Comparative Pathology, Vol. 118, No. 1, 1998, p. 15-27.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In-situ apoptosis in chickens infected with infectious bursal disease virus
AU - Tanimura, N.
AU - Sharma, J. M.
PY - 1998
Y1 - 1998
N2 - Groups of specific pathogen-free chickens aged 3 weeks were inoculated with one of three strains of infectious bursal disease virus (IBDV), namely: (1) a classical virulent strain, IM-IBDV; (2) an antigenic variant-E strain, VE-IBDV; and (3) an attenuated vaccine strain, B2-IBDV. The bursa of Fabricius and thymus were examined 1 to 5 days after inoculation for histopathological lesions, IBDV antigen, and in-situ apoptosis. Infection of chickens with IM-, VE-, or B2-IBDV resulted in the appearance of large numbers of apoptotic bursal lymphocytes, both in IBDV antigen-positive and antigen-negative bursal follicles. B2-IBDV induced a slower increase in the IBDV antigen-positive cells and apoptotic cells than did IM- and VE-IBDV. The results suggested that virulent strains and vaccine strains of IBDV enhance endogenous apoptosis of bursal lymphocytes that are free of detectable IBDV antigen. Infection of chickens with IM-IBDV, but not with VE- or B2-IBDV, caused a significant increase in apoptotic cells in the thymic cortical lymphocytes. The apoptotic lymphocytes in the thymus were negative for IBDV antigen. This result indicated that histopathological lesions in the thymus of IM-IBDV-infected chickens may be due to enhanced endogenous apoptosis of thymic lymphocytes without IBDV antigen production in the cells.
AB - Groups of specific pathogen-free chickens aged 3 weeks were inoculated with one of three strains of infectious bursal disease virus (IBDV), namely: (1) a classical virulent strain, IM-IBDV; (2) an antigenic variant-E strain, VE-IBDV; and (3) an attenuated vaccine strain, B2-IBDV. The bursa of Fabricius and thymus were examined 1 to 5 days after inoculation for histopathological lesions, IBDV antigen, and in-situ apoptosis. Infection of chickens with IM-, VE-, or B2-IBDV resulted in the appearance of large numbers of apoptotic bursal lymphocytes, both in IBDV antigen-positive and antigen-negative bursal follicles. B2-IBDV induced a slower increase in the IBDV antigen-positive cells and apoptotic cells than did IM- and VE-IBDV. The results suggested that virulent strains and vaccine strains of IBDV enhance endogenous apoptosis of bursal lymphocytes that are free of detectable IBDV antigen. Infection of chickens with IM-IBDV, but not with VE- or B2-IBDV, caused a significant increase in apoptotic cells in the thymic cortical lymphocytes. The apoptotic lymphocytes in the thymus were negative for IBDV antigen. This result indicated that histopathological lesions in the thymus of IM-IBDV-infected chickens may be due to enhanced endogenous apoptosis of thymic lymphocytes without IBDV antigen production in the cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031936665&partnerID=8YFLogxK
M3 - Article
VL - 118
SP - 15
EP - 27
JO - Journal of Comparative Pathology
JF - Journal of Comparative Pathology
SN - 0021-9975
IS - 1
ER -