In-situ apoptosis in chickens infected with infectious bursal disease virus

N. Tanimura, J. M. Sharma

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Groups of specific pathogen-free chickens aged 3 weeks were inoculated with one of three strains of infectious bursal disease virus (IBDV), namely: (1) a classical virulent strain, IM-IBDV; (2) an antigenic variant-E strain, VE-IBDV; and (3) an attenuated vaccine strain, B2-IBDV. The bursa of Fabricius and thymus were examined 1 to 5 days after inoculation for histopathological lesions, IBDV antigen, and in-situ apoptosis. Infection of chickens with IM-, VE-, or B2-IBDV resulted in the appearance of large numbers of apoptotic bursal lymphocytes, both in IBDV antigen-positive and antigen-negative bursal follicles. B2-IBDV induced a slower increase in the IBDV antigen-positive cells and apoptotic cells than did IM- and VE-IBDV. The results suggested that virulent strains and vaccine strains of IBDV enhance endogenous apoptosis of bursal lymphocytes that are free of detectable IBDV antigen. Infection of chickens with IM-IBDV, but not with VE- or B2-IBDV, caused a significant increase in apoptotic cells in the thymic cortical lymphocytes. The apoptotic lymphocytes in the thymus were negative for IBDV antigen. This result indicated that histopathological lesions in the thymus of IM-IBDV-infected chickens may be due to enhanced endogenous apoptosis of thymic lymphocytes without IBDV antigen production in the cells.

Original languageEnglish (US)
Pages (from-to)15-27
Number of pages13
JournalJournal of Comparative Pathology
Volume118
Issue number1
StatePublished - 1998
Externally publishedYes

Fingerprint

Infectious bursal disease virus
Chickens
apoptosis
Apoptosis
chickens
antigens
Antigens
lymphocytes
Lymphocytes
Thymus Gland
lesions (animal)
cells
Bursa of Fabricius
Specific Pathogen-Free Organisms
Attenuated Vaccines

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

In-situ apoptosis in chickens infected with infectious bursal disease virus. / Tanimura, N.; Sharma, J. M.

In: Journal of Comparative Pathology, Vol. 118, No. 1, 1998, p. 15-27.

Research output: Contribution to journalArticle

Tanimura, N. ; Sharma, J. M. / In-situ apoptosis in chickens infected with infectious bursal disease virus. In: Journal of Comparative Pathology. 1998 ; Vol. 118, No. 1. pp. 15-27.
@article{1be2958baec2452a81ac73548fd99926,
title = "In-situ apoptosis in chickens infected with infectious bursal disease virus",
abstract = "Groups of specific pathogen-free chickens aged 3 weeks were inoculated with one of three strains of infectious bursal disease virus (IBDV), namely: (1) a classical virulent strain, IM-IBDV; (2) an antigenic variant-E strain, VE-IBDV; and (3) an attenuated vaccine strain, B2-IBDV. The bursa of Fabricius and thymus were examined 1 to 5 days after inoculation for histopathological lesions, IBDV antigen, and in-situ apoptosis. Infection of chickens with IM-, VE-, or B2-IBDV resulted in the appearance of large numbers of apoptotic bursal lymphocytes, both in IBDV antigen-positive and antigen-negative bursal follicles. B2-IBDV induced a slower increase in the IBDV antigen-positive cells and apoptotic cells than did IM- and VE-IBDV. The results suggested that virulent strains and vaccine strains of IBDV enhance endogenous apoptosis of bursal lymphocytes that are free of detectable IBDV antigen. Infection of chickens with IM-IBDV, but not with VE- or B2-IBDV, caused a significant increase in apoptotic cells in the thymic cortical lymphocytes. The apoptotic lymphocytes in the thymus were negative for IBDV antigen. This result indicated that histopathological lesions in the thymus of IM-IBDV-infected chickens may be due to enhanced endogenous apoptosis of thymic lymphocytes without IBDV antigen production in the cells.",
author = "N. Tanimura and Sharma, {J. M.}",
year = "1998",
language = "English (US)",
volume = "118",
pages = "15--27",
journal = "Journal of Comparative Pathology",
issn = "0021-9975",
publisher = "W.B. Saunders Ltd",
number = "1",

}

TY - JOUR

T1 - In-situ apoptosis in chickens infected with infectious bursal disease virus

AU - Tanimura, N.

AU - Sharma, J. M.

PY - 1998

Y1 - 1998

N2 - Groups of specific pathogen-free chickens aged 3 weeks were inoculated with one of three strains of infectious bursal disease virus (IBDV), namely: (1) a classical virulent strain, IM-IBDV; (2) an antigenic variant-E strain, VE-IBDV; and (3) an attenuated vaccine strain, B2-IBDV. The bursa of Fabricius and thymus were examined 1 to 5 days after inoculation for histopathological lesions, IBDV antigen, and in-situ apoptosis. Infection of chickens with IM-, VE-, or B2-IBDV resulted in the appearance of large numbers of apoptotic bursal lymphocytes, both in IBDV antigen-positive and antigen-negative bursal follicles. B2-IBDV induced a slower increase in the IBDV antigen-positive cells and apoptotic cells than did IM- and VE-IBDV. The results suggested that virulent strains and vaccine strains of IBDV enhance endogenous apoptosis of bursal lymphocytes that are free of detectable IBDV antigen. Infection of chickens with IM-IBDV, but not with VE- or B2-IBDV, caused a significant increase in apoptotic cells in the thymic cortical lymphocytes. The apoptotic lymphocytes in the thymus were negative for IBDV antigen. This result indicated that histopathological lesions in the thymus of IM-IBDV-infected chickens may be due to enhanced endogenous apoptosis of thymic lymphocytes without IBDV antigen production in the cells.

AB - Groups of specific pathogen-free chickens aged 3 weeks were inoculated with one of three strains of infectious bursal disease virus (IBDV), namely: (1) a classical virulent strain, IM-IBDV; (2) an antigenic variant-E strain, VE-IBDV; and (3) an attenuated vaccine strain, B2-IBDV. The bursa of Fabricius and thymus were examined 1 to 5 days after inoculation for histopathological lesions, IBDV antigen, and in-situ apoptosis. Infection of chickens with IM-, VE-, or B2-IBDV resulted in the appearance of large numbers of apoptotic bursal lymphocytes, both in IBDV antigen-positive and antigen-negative bursal follicles. B2-IBDV induced a slower increase in the IBDV antigen-positive cells and apoptotic cells than did IM- and VE-IBDV. The results suggested that virulent strains and vaccine strains of IBDV enhance endogenous apoptosis of bursal lymphocytes that are free of detectable IBDV antigen. Infection of chickens with IM-IBDV, but not with VE- or B2-IBDV, caused a significant increase in apoptotic cells in the thymic cortical lymphocytes. The apoptotic lymphocytes in the thymus were negative for IBDV antigen. This result indicated that histopathological lesions in the thymus of IM-IBDV-infected chickens may be due to enhanced endogenous apoptosis of thymic lymphocytes without IBDV antigen production in the cells.

UR - http://www.scopus.com/inward/record.url?scp=0031936665&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031936665&partnerID=8YFLogxK

M3 - Article

C2 - 9500235

AN - SCOPUS:0031936665

VL - 118

SP - 15

EP - 27

JO - Journal of Comparative Pathology

JF - Journal of Comparative Pathology

SN - 0021-9975

IS - 1

ER -