In search of RNase P RNA from microbial genomes

Yong Li, Sidney Altman

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A simple procedure has been developed to quickly retrieve and validate the DNA sequence encoding the RNA subunit of ribonuclease P (RNase P RNA) from microbial genomes. RNase P RNA sequences were identified from 94% of bacterial and archaeal complete genomes where previously no RNase P RNA was annotated. A sequence was found in camelpox virus, highly conserved in all orthopoxviruses (including smallpox virus), which could fold into a putative RNase P RNA in terms of conserved primary features and secondary structure. New structure features of RNase P RNA that enable one to distinguish bacteria from archaea and eukarya were found. This RNA is yet another RNA that can be a molecular criterion to divide the living world into three domains (bacteria, archaea, and eukarya). The catalytic center of this RNA, and its detection from some environmental whole genome shotgun sequences, is also discussed.

Original languageEnglish (US)
Pages (from-to)1533-1540
Number of pages8
JournalRNA
Volume10
Issue number10
DOIs
StatePublished - Oct 2004
Externally publishedYes

Fingerprint

Microbial Genome
Ribonuclease P
RNA
Orthopoxvirus
Archaea
Eukaryota
Archaeal Genome
Variola virus
Bacteria
Catalytic RNA
Firearms
Genome

Keywords

  • Camelpox virus
  • Core structure
  • Pattern recognition sequence
  • Preprocessing programs

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

In search of RNase P RNA from microbial genomes. / Li, Yong; Altman, Sidney.

In: RNA, Vol. 10, No. 10, 10.2004, p. 1533-1540.

Research output: Contribution to journalArticle

Li, Yong ; Altman, Sidney. / In search of RNase P RNA from microbial genomes. In: RNA. 2004 ; Vol. 10, No. 10. pp. 1533-1540.
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