In Cellulo Synthesis of Proteins Containing a Fluorescent Oxazole Amino Acid

Shengxi Chen, Xun Ji, Mingxuan Gao, Larisa Dedkova, Sidney Hecht

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Genetic code expansion has enabled many noncanonical amino acids to be incorporated into proteins in vitro and in cellulo. These have largely involved α-l-amino acids, reflecting the substrate specificity of natural aminoacyl-tRNA synthetases and ribosomes. Recently, modified E. coli ribosomes, selected using a dipeptidylpuromycin analogue, were employed to incorporate dipeptides and dipeptidomimetics. Presently, we report the in cellulo incorporation of a strongly fluorescent oxazole amino acid (lacking an asymmetric center or α-amino group) by using modified ribosomes and pyrrolysyl-tRNA synthetase (PylRS). Initially, a plasmid encoding the RRM1 domain of putative transcription factor hnRNP LL was cotransformed with plasmid pTECH-Pyl-OP in E. coli cells, having modified ribosomes able to incorporate dipeptides. Cell incubation in a medium containing oxazole 2 resulted in the elaboration of RRM1 containing the oxazole. Green fluorescent protein, previously expressed in vitro with several different oxazole amino acids at position 66, was also expressed in cellulo containing oxazole 2; the incorporation was verified by mass spectrometry. Finally, oxazole 2 was incorporated into position 13 of MreB, a bacterial homologue of eukaryotic cytoskeletal protein actin F. Modified MreB expressed in vitro and in cellulo comigrated with wild type. E. coli cells expressing the modified MreB were strongly fluorescent and retained the E. coli cell rod-like phenotype. For each protein studied, the incorporation of oxazole 2 strongly increased oxazole fluorescence, suggesting its potential utility as a protein tag. These findings also suggest the feasibility of dramatically increasing the repertoire of amino acids that can be genetically encoded for protein incorporation in cellulo.

Original languageEnglish (US)
Pages (from-to)5597-5601
Number of pages5
JournalJournal of the American Chemical Society
Volume141
Issue number14
DOIs
StatePublished - Apr 10 2019

Fingerprint

Oxazoles
Amino acids
Proteins
Amino Acids
Escherichia coli
Ribosomes
Amino Acyl-tRNA Synthetases
Dipeptides
Plasmids
Transcription factors
Heterogeneous-Nuclear Ribonucleoproteins
Genetic Code
Cytoskeletal Proteins
Mass spectrometry
Substrate Specificity
Green Fluorescent Proteins
Fluorescence
Cells
Actins
Mass Spectrometry

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

In Cellulo Synthesis of Proteins Containing a Fluorescent Oxazole Amino Acid. / Chen, Shengxi; Ji, Xun; Gao, Mingxuan; Dedkova, Larisa; Hecht, Sidney.

In: Journal of the American Chemical Society, Vol. 141, No. 14, 10.04.2019, p. 5597-5601.

Research output: Contribution to journalArticle

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