Improving genetic transformation rates in honeybees

M. Otte, O. Netschitailo, O. Kaftanoglu, Y. Wang, Robert Page, M. Beye

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Functional genetic studies in honeybees have been limited by transformation tools that lead to a high rate of transposon integration into the germline of the queens. A high transformation rate is required to reduce screening efforts because each treated queen needs to be maintained in a separate honeybee colony. Here, we report on further improvement of the transformation rate in honeybees by using a combination of different procedures. We employed a hyperactive transposase protein (hyPBaseapis), we tripled the amount of injected transposase mRNAs and we injected embryos into the first third (anterior part) of the embryo. These three improvements together doubled the transformation rate from 19% to 44%. We propose that the hyperactive transposase (hyPBaseapis) and the other steps used may also help to improve the transformation rates in other species in which screening and crossing procedures are laborious.

Original languageEnglish (US)
Article number16534
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Transposases
Genetic Transformation
Embryonic Structures
Messenger RNA
Proteins

ASJC Scopus subject areas

  • General

Cite this

Otte, M., Netschitailo, O., Kaftanoglu, O., Wang, Y., Page, R., & Beye, M. (2018). Improving genetic transformation rates in honeybees. Scientific Reports, 8(1), [16534]. https://doi.org/10.1038/s41598-018-34724-w

Improving genetic transformation rates in honeybees. / Otte, M.; Netschitailo, O.; Kaftanoglu, O.; Wang, Y.; Page, Robert; Beye, M.

In: Scientific Reports, Vol. 8, No. 1, 16534, 01.12.2018.

Research output: Contribution to journalArticle

Otte, M, Netschitailo, O, Kaftanoglu, O, Wang, Y, Page, R & Beye, M 2018, 'Improving genetic transformation rates in honeybees', Scientific Reports, vol. 8, no. 1, 16534. https://doi.org/10.1038/s41598-018-34724-w
Otte M, Netschitailo O, Kaftanoglu O, Wang Y, Page R, Beye M. Improving genetic transformation rates in honeybees. Scientific Reports. 2018 Dec 1;8(1). 16534. https://doi.org/10.1038/s41598-018-34724-w
Otte, M. ; Netschitailo, O. ; Kaftanoglu, O. ; Wang, Y. ; Page, Robert ; Beye, M. / Improving genetic transformation rates in honeybees. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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