Abstract

A recently developed dynamic fluorescence immunoassay for the biomarker Myoglobin promises to provide detection of myocardial infarction within minutes. Signal extraction for this method is based on epifluorescence video microscopy, but is confounded by inhomogeneous spatial gain, intensity inhomogeneity or IIH. We present here a novel adaptive correction method which estimates the IIH from a video image sequence. This estimated IIH may be used to correct the image signal extraction process. The enhancement to detection limits and consistency of estimated concentrations are quantified, based on an improvement to SNR of approximately 9 dB.

Original languageEnglish (US)
Title of host publication2008 42nd Asilomar Conference on Signals, Systems and Computers, ASILOMAR 2008
Pages1975-1978
Number of pages4
DOIs
StatePublished - Dec 1 2008
Event2008 42nd Asilomar Conference on Signals, Systems and Computers, ASILOMAR 2008 - Pacific Grove, CA, United States
Duration: Oct 26 2008Oct 29 2008

Publication series

NameConference Record - Asilomar Conference on Signals, Systems and Computers
ISSN (Print)1058-6393

Other

Other2008 42nd Asilomar Conference on Signals, Systems and Computers, ASILOMAR 2008
CountryUnited States
CityPacific Grove, CA
Period10/26/0810/29/08

ASJC Scopus subject areas

  • Signal Processing
  • Computer Networks and Communications

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    Mahanti, P., Taylor, T., Cochran, D., Hayes, M., & Petkus, M. (2008). Improved signal extraction from fluorescence immunoassay image sequences. In 2008 42nd Asilomar Conference on Signals, Systems and Computers, ASILOMAR 2008 (pp. 1975-1978). [5074776] (Conference Record - Asilomar Conference on Signals, Systems and Computers). https://doi.org/10.1109/ACSSC.2008.5074776