TY - GEN
T1 - Improved signal extraction from fluorescence immunoassay image sequences
AU - Mahanti, Prasun
AU - Taylor, Thomas
AU - Cochran, Douglas
AU - Hayes, Mark
AU - Petkus, Mathew
PY - 2008
Y1 - 2008
N2 - A recently developed dynamic fluorescence immunoassay for the biomarker Myoglobin promises to provide detection of myocardial infarction within minutes. Signal extraction for this method is based on epifluorescence video microscopy, but is confounded by inhomogeneous spatial gain, intensity inhomogeneity or IIH. We present here a novel adaptive correction method which estimates the IIH from a video image sequence. This estimated IIH may be used to correct the image signal extraction process. The enhancement to detection limits and consistency of estimated concentrations are quantified, based on an improvement to SNR of approximately 9 dB.
AB - A recently developed dynamic fluorescence immunoassay for the biomarker Myoglobin promises to provide detection of myocardial infarction within minutes. Signal extraction for this method is based on epifluorescence video microscopy, but is confounded by inhomogeneous spatial gain, intensity inhomogeneity or IIH. We present here a novel adaptive correction method which estimates the IIH from a video image sequence. This estimated IIH may be used to correct the image signal extraction process. The enhancement to detection limits and consistency of estimated concentrations are quantified, based on an improvement to SNR of approximately 9 dB.
UR - http://www.scopus.com/inward/record.url?scp=70349670989&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349670989&partnerID=8YFLogxK
U2 - 10.1109/ACSSC.2008.5074776
DO - 10.1109/ACSSC.2008.5074776
M3 - Conference contribution
AN - SCOPUS:70349670989
SN - 9781424429417
T3 - Conference Record - Asilomar Conference on Signals, Systems and Computers
SP - 1975
EP - 1978
BT - 2008 42nd Asilomar Conference on Signals, Systems and Computers, ASILOMAR 2008
T2 - 2008 42nd Asilomar Conference on Signals, Systems and Computers, ASILOMAR 2008
Y2 - 26 October 2008 through 29 October 2008
ER -