Abstract

A recently developed dynamic fluorescence immunoassay for the biomarker Myoglobin promises to provide detection of myocardial infarction within minutes. Signal extraction for this method is based on epifluorescence video microscopy, but is confounded by inhomogeneous spatial gain, intensity inhomogeneity or IIH. We present here a novel adaptive correction method which estimates the IIH from a video image sequence. This estimated IIH may be used to correct the image signal extraction process. The enhancement to detection limits and consistency of estimated concentrations are quantified, based on an improvement to SNR of approximately 9 dB.

Original languageEnglish (US)
Title of host publicationConference Record - Asilomar Conference on Signals, Systems and Computers
Pages1975-1978
Number of pages4
DOIs
StatePublished - 2008
Event2008 42nd Asilomar Conference on Signals, Systems and Computers, ASILOMAR 2008 - Pacific Grove, CA, United States
Duration: Oct 26 2008Oct 29 2008

Other

Other2008 42nd Asilomar Conference on Signals, Systems and Computers, ASILOMAR 2008
CountryUnited States
CityPacific Grove, CA
Period10/26/0810/29/08

Fingerprint

Fluorescence
Biomarkers
Microscopic examination

ASJC Scopus subject areas

  • Computer Networks and Communications
  • Signal Processing

Cite this

Mahanti, P., Taylor, T., Cochran, D., Hayes, M., & Petkus, M. (2008). Improved signal extraction from fluorescence immunoassay image sequences. In Conference Record - Asilomar Conference on Signals, Systems and Computers (pp. 1975-1978). [5074776] https://doi.org/10.1109/ACSSC.2008.5074776

Improved signal extraction from fluorescence immunoassay image sequences. / Mahanti, Prasun; Taylor, Thomas; Cochran, Douglas; Hayes, Mark; Petkus, Mathew.

Conference Record - Asilomar Conference on Signals, Systems and Computers. 2008. p. 1975-1978 5074776.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Mahanti, P, Taylor, T, Cochran, D, Hayes, M & Petkus, M 2008, Improved signal extraction from fluorescence immunoassay image sequences. in Conference Record - Asilomar Conference on Signals, Systems and Computers., 5074776, pp. 1975-1978, 2008 42nd Asilomar Conference on Signals, Systems and Computers, ASILOMAR 2008, Pacific Grove, CA, United States, 10/26/08. https://doi.org/10.1109/ACSSC.2008.5074776
Mahanti P, Taylor T, Cochran D, Hayes M, Petkus M. Improved signal extraction from fluorescence immunoassay image sequences. In Conference Record - Asilomar Conference on Signals, Systems and Computers. 2008. p. 1975-1978. 5074776 https://doi.org/10.1109/ACSSC.2008.5074776
Mahanti, Prasun ; Taylor, Thomas ; Cochran, Douglas ; Hayes, Mark ; Petkus, Mathew. / Improved signal extraction from fluorescence immunoassay image sequences. Conference Record - Asilomar Conference on Signals, Systems and Computers. 2008. pp. 1975-1978
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