Improved isolation and crystallization of Photosystem I for structural analysis

Petra Fromme, Horst Tobias Witt

Research output: Contribution to journalArticlepeer-review

107 Scopus citations

Abstract

Stable trimeric Photosystem I (PS I) was isolated from the cyanobacterium Synechococcus elongatus by use of stereochemically pure β- dodecylmaltoside. Crystals of extremely pure PSI are grown by dialysis against low salt concentration. The improved PS I crystals were the basis for a PSI model derived from X-ray structure analysis at 4 resolution (Krauss et al., Nat. Struct. Biol. 3 (1996) 965-973; Schubert et al., J. Mol. Biol. 272 (1997) 741-769) and for detailed information on the electron transfer chain elaborated by techniques of magnetic resonance (H. Kass, Thesis, TU-Berlin, 1995; Kamlowski et al., Biochim. Biophys. Acta 1319 (1997) 188-198 and 199- 213; Bittl et al., Biochemistry 36 (1997) 12001-12004). Crystallization procedures using micro- and macroseeding are described as basis for the forthcoming structure analysis.

Original languageEnglish (US)
Pages (from-to)175-184
Number of pages10
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1365
Issue number1-2
DOIs
StatePublished - Jun 10 1998
Externally publishedYes

Keywords

  • Crystallization
  • Membrane protein
  • Photosynthesis
  • Photosystem I
  • Structure analysis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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