Improved affinity selection using phage display technology and off-rate based selection

Bin Yuan, Philip Schulz, Ruitian Liu, Michael Sierks

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Flow systems such as a BIAcore biosensor can be very efficient tools to isolate high affinity antibody fragments from affinity matured phage display libraries. Here we show that using flow based selection, we can readily isolate a variant with a 35-fold higher affinity, especially with a 7 fold better off-rate, compared to the parent clone after only a single round of selection from a second generation affinity matured phage display library. The flow system represents a fast method to isolate affinity improved antibody fragments and can be particularly useful for isolating antibodies to antigens that have poor solubility, are toxic to the host cell, or prone to aggregation.

Original languageEnglish (US)
Pages (from-to)171-175
Number of pages5
JournalElectronic Journal of Biotechnology
Volume9
Issue number2
DOIs
StatePublished - Apr 15 2006

Fingerprint

Immunoglobulin Fragments
Bacteriophages
Antibodies
Libraries
Display devices
Technology
Antibody Affinity
Poisons
Biosensing Techniques
Biosensors
Solubility
Agglomeration
Clone Cells
Antigens

Keywords

  • β-amyloid (Aβ)
  • Affinity maturation
  • Dissociation rate
  • Phage display

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Improved affinity selection using phage display technology and off-rate based selection. / Yuan, Bin; Schulz, Philip; Liu, Ruitian; Sierks, Michael.

In: Electronic Journal of Biotechnology, Vol. 9, No. 2, 15.04.2006, p. 171-175.

Research output: Contribution to journalArticle

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