Abstract

To examine the impact of environmental factors on Legionella in drinking water distribution systems, the growth and survival of Legionella under various conditions was studied. When incubated in tap water at 4 °C, 25 °C, and 32 °C, L. pneumophila survival trends varied amongst the temperatures, with the stable populations maintained for months at 25 °C and 32 °C demonstrating that survival is possible at these temperatures for extended periods in oligotrophic conditions. After inoculating coupons of PVC, copper, brass, and cast iron, L. pneumophila colonized biofilms formed on each within days to a similar extent, with the exception of cast iron, which contained 1-log less Legionella after 90 days. L. pneumophila spiked in a model drinking water distribution system colonized the system within days. Chlorination of the system had a greater effect on biofilm-associated Legionella concentrations, with populations returning to pre-chlorination levels within six weeks. Biofilms sampled from drinking water meters collected from two areas within central Arizona were analyzed via PCR for the presence of Legionella. Occurrence in only one area indicates that environmental differences in water distribution systems may have an impact on the survival of Legionella. These esults document the impact of different environmental conditions on the survival of Legionella in water.

Original languageEnglish (US)
Pages (from-to)269-282
Number of pages14
JournalPathogens
Volume4
Issue number2
DOIs
StatePublished - May 19 2015

Fingerprint

Legionella
Drinking Water
Population
Biofilms
Halogenation
Water
Iron
Temperature
Population Dynamics
Polyvinyl Chloride
Copper
Polymerase Chain Reaction
Growth

Keywords

  • Biofilm
  • Distribution system
  • Drinking water
  • Legionella pneumophila
  • Survival
  • Temperature

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Microbiology (medical)
  • Immunology and Allergy
  • Molecular Biology

Cite this

Impact of environmental factors on legionella populations in drinking water. / Schwake, David Otto; Alum, Absar; Abbaszadegan, Morteza.

In: Pathogens, Vol. 4, No. 2, 19.05.2015, p. 269-282.

Research output: Contribution to journalArticle

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