Impact of artifactual Ex Vivo oxidation on biochemical research

Chad Borges, Joshua W. Jeffs, Erandi P. Kapuruge

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Once exposed to the atmosphere all major classes of bio-organic molecules are susceptible to oxidation that they would not normally experience in vivo. Direct reactions with oxygen are spin forbidden, but frequently unavoidable trace quantities of redox-active transition metals are often thermodynamically positioned to facilitate formation of biomolecular radicals and/or formation of superoxide radicals, creating a stream of reactive oxygen species (ROS) that readily damage biomolecules. Generally the degree of damage depends on the availability of O2, concentration of redox active metals, temperature, and the length of exposure above the freezing point of the specimen. Using examples from the biomedical literature, this chapter provides an overview of the ways in which DNA, proteins, and lipids can experience artifactual oxidation ex vivo and the potential impact these reactions may have on the goal(s) of an investigation.

Original languageEnglish (US)
Title of host publicationOxidative Stress: Diagnostics, Prevention, and Therapy Volume 2
PublisherAmerican Chemical Society
Pages375-413
Number of pages39
Volume1200
ISBN (Print)9780841231009
DOIs
StatePublished - 2015

Publication series

NameACS Symposium Series
Volume1200
ISSN (Print)00976156
ISSN (Electronic)19475918

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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  • Cite this

    Borges, C., Jeffs, J. W., & Kapuruge, E. P. (2015). Impact of artifactual Ex Vivo oxidation on biochemical research. In Oxidative Stress: Diagnostics, Prevention, and Therapy Volume 2 (Vol. 1200, pp. 375-413). (ACS Symposium Series; Vol. 1200). American Chemical Society. https://doi.org/10.1021/bk-2015-1200.ch016