Impact of acetaminophen consumption and resistance exercise on extracellular matrix gene expression in human skeletal muscle

Shivam H. Patel, Andrew C. D’Lugos, Erica R. Eldon, Donald Curtis, Jared Dickinson, Chad C. Carroll

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Acetaminophen (APAP) given during chronic exercise reduces skeletal muscle collagen and cross-linking in rats. We propose that the effect of APAP on muscle extracellular matrix (ECM) may, in part, be mediated by dysregulation of the balance between matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs). The purpose of this study was to evaluate the impact of APAP consumption during acute resistance exercise (RE) on several regulators of the ECM in human skeletal muscle. In a double-blinded, placebo-controlled, randomized crossover design, recreationally active men (n = 8, 25 ± 2 yr) performed two trials of knee extension. Placebo (PLA) or APAP (1,000 mg/6 h) was given for 24 h before and immediately following RE. Vastus lateralis biopsies were taken at baseline and 1 and 3 h post-RE. Quantitative RT-PCR was used to determine differences in mRNA expression. MMP-2, type I collagen, and type III collagen mRNA expression was not altered by exercise or APAP (P > 0.05). When compared with PLA, TIMP-1 expression was lower at 1 h post-RE during APAP conditions but greater than PLA at 3 h post-RE (P < 0.05). MMP-9 expression and protein levels were elevated at 3 h post-RE independent of treatment (P < 0.05). Lysyl oxidase expression was greater at 3 h post-RE during APAP consumption (P < 0.05) compared with PLA. MMP-2 and TIMP-1 protein was not altered by RE or APAP (P > 0.05). Phosphorylation of ERK1/2 and p38-MAPK increased (P < 0.05) with RE but was not influenced by APAP. Our findings do not support our hypothesis and suggest that short-term APAP consumption before RE has a small impact on the measured ECM molecules in human skeletal muscle following acute RE.

Original languageEnglish (US)
Pages (from-to)R44-R50
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume313
Issue number1
DOIs
StatePublished - 2017

Fingerprint

Acetaminophen
Extracellular Matrix
Skeletal Muscle
Exercise
Gene Expression
Placebos
Matrix Metalloproteinase Inhibitors
Collagen
Tissue Inhibitor of Metalloproteinases
Messenger RNA
Collagen Type III
Matrix Metalloproteinase 2
Quadriceps Muscle
p38 Mitogen-Activated Protein Kinases
Matrix Metalloproteinases
Cross-Over Studies
Knee
Phosphorylation
Biopsy
Muscles

Keywords

  • Exercise
  • Extracellular matrix
  • Paracetamol

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Impact of acetaminophen consumption and resistance exercise on extracellular matrix gene expression in human skeletal muscle. / Patel, Shivam H.; D’Lugos, Andrew C.; Eldon, Erica R.; Curtis, Donald; Dickinson, Jared; Carroll, Chad C.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 313, No. 1, 2017, p. R44-R50.

Research output: Contribution to journalArticle

Patel, Shivam H. ; D’Lugos, Andrew C. ; Eldon, Erica R. ; Curtis, Donald ; Dickinson, Jared ; Carroll, Chad C. / Impact of acetaminophen consumption and resistance exercise on extracellular matrix gene expression in human skeletal muscle. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2017 ; Vol. 313, No. 1. pp. R44-R50.
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