Immunohistochemical characterization of clathrin assembly protein AP180 and synaptophysin in human brain

Pamela J. Yao, Timothy M. O'Herron, Paul Coleman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Neurons rely on clathrin-mediated endocytosis for retrieving synaptic vesicles (SVs) at the presynaptic compartment after the release of neurotransmitters. The clathrin assembly protein AP180 is shown to be a regulator for this clathrin-dependent SV recycling pathway. AP180 is efficient in facilitating the formation of clathrin-coated vesicles and regulating their size, but its exact location in synapse is not clear. In this study, we compared the expression of AP180 with synaptophysin in the aged human brain using confocal immunofluorescence microscopy. Synaptophysin is well characterized for its association with SVs and therefore a commonly used presynaptic marker. We achieved satisfactory immunofluorescent labeling by using an autofluorescence blocker Sudan Black B and more photostable Alexa Fluor dyes. Although we found that AP180 had an overall expression similar to synaptophysin, the immunoreactivity for the two proteins did not always co-localize.

Original languageEnglish (US)
Pages (from-to)173-178
Number of pages6
JournalNeurobiology of Aging
Volume24
Issue number1
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

Fingerprint

Synaptophysin
Synaptic Vesicles
Clathrin
Brain
Clathrin-Coated Vesicles
Endocytosis
Fluorescence Microscopy
Confocal Microscopy
Synapses
Neurotransmitter Agents
Coloring Agents
Neurons
clathrin assembly protein AP180
Proteins

Keywords

  • Alexa Fluor dyes
  • AP180
  • Autofluorescence
  • Clathrin assembly protein
  • Sudan Black B
  • Synapse
  • Synaptic vesicle recycling
  • Synaptophysin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Aging
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology

Cite this

Immunohistochemical characterization of clathrin assembly protein AP180 and synaptophysin in human brain. / Yao, Pamela J.; O'Herron, Timothy M.; Coleman, Paul.

In: Neurobiology of Aging, Vol. 24, No. 1, 01.01.2003, p. 173-178.

Research output: Contribution to journalArticle

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