Immunization of pigs by DNA prime and recombinant vaccinia virus boost to identify and rank African swine fever virus immunogenic and protective proteins

James K. Jancovich, Dave Chapman, Debra Hansen, Mark D. Robida, Andrey Loskutov, Felicia Craciunescu, Alex Borovkov, Karen Kibler, Lynnette Goatley, Katherine King, Christopher L. Netherton, Geraldine Taylor, Bertram Jacobs, Kathryn Sykes, Linda K. Dixon

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13 Scopus citations

Abstract

African swine fever virus (ASFV) causes an acute hemorrhagic fever in domestic pigs, with high socioeconomic impact. No vaccine is available, limiting options for control. Although live attenuated ASFV can induce up to 100% protection against lethal challenge, little is known of the antigens which induce this protective response. To identify additional ASFV immunogenic and potentially protective antigens, we cloned 47 viral genes in individual plasmids for gene vaccination and in recombinant vaccinia viruses. These antigens were selected to include proteins with different functions and timing of expression. Pools of up to 22 antigens were delivered by DNA prime and recombinant vaccinia virus boost to groups of pigs. Responses of immune lymphocytes from pigs to individual recombinant proteins and to ASFV were measured by interferon gamma enzyme-linked immunosorbent spot (ELISpot) assays to identify a subset of the antigens that consistently induced the highest responses. All 47 antigens were then delivered to pigs by DNA prime and recombinant vaccinia virus boost, and pigs were challenged with a lethal dose of ASFV isolate Georgia 2007/1. Although pigs developed clinical and pathological signs consistent with acute ASFV, viral genome levels were significantly reduced in blood and several lymph tissues in those pigs immunized with vectors expressing ASFV antigens compared with the levels in control pigs.

Original languageEnglish (US)
Article numbere02219-17
JournalJournal of Virology
Volume92
Issue number8
DOIs
StatePublished - Apr 1 2018

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Keywords

  • African swine fever virus
  • DNA vaccines
  • Immune markers
  • Vaccinia virus
  • Veterinary vaccine development

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Jancovich, J. K., Chapman, D., Hansen, D., Robida, M. D., Loskutov, A., Craciunescu, F., Borovkov, A., Kibler, K., Goatley, L., King, K., Netherton, C. L., Taylor, G., Jacobs, B., Sykes, K., & Dixon, L. K. (2018). Immunization of pigs by DNA prime and recombinant vaccinia virus boost to identify and rank African swine fever virus immunogenic and protective proteins. Journal of Virology, 92(8), [e02219-17]. https://doi.org/10.1128/JVI.02219-17